Frequency of HLA-G exon 8 polymorphisms and kidney allograft outcome in Iranian population

• Published in: Molecular biology reports 2011-06, Vol.38 (5), p.3593-3597
• Main Authors: Aghdaie, Mahdokht H., Azarpira, Negar, Kazemi, Kurosh, Geramizadeh, Bita, Darai, Masumeh, Malekhoseini, Seid Ali
• Format: Article
• Language: English
• Subjects: Adult; Animal Anatomy; Animal Biochemistry; Biomedical and Life Sciences; Exons; Female; Genetic markers; Genotype; Genotype & phenotype; Graft Rejection - genetics; Graft Rejection - immunology; Histocompatibility Antigens Class I - genetics; Histology; HLA Antigens - genetics; HLA-G Antigens; Humans; Iran; Kidney Transplantation - immunology; Kidneys; Life Sciences; Male; Molecular biology; Morphology; Polymorphism; Polymorphism, Genetic; RNA Stability - genetics; Transplantation, Homologous - immunology; Transplants & implants; Treatment Outcome; Young Adult
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-010-0470-y

The 14-bp polymorphism in exon 8 of the HLA-G gene is associated with HLA-G mRNA stability and the patterns of alternative isoform splicing and may influence the functionality of the HLA-G molecule. HLA-G expression was related to allograft acceptance and fewer episodes of acute rejection during heart, kidney and liver–kidney transplantation. In order to determine a possible correlation between the 14-bp insertion/deletion polymorphism and kidney allograft outcome in our population, genomic DNA was isolated from 144 patients who had received isolated kidney allografts. The recipients was divided into two groups, grafts presenting features of rejection group and a non-rejection group, and compared them with a control group of 100 healthy subjects. There was no significant difference in allelic frequencies of 14-bp insertion/deletion polymorphism between normal controls and kidney transplant patients. No significant difference was found between the RG and the NRG regarding the 14-bp genotypes and alleles. Therefore, additional studies with more sample size from other populations with analysis of other HLA-G polymorphisms are necessary to define this polymorphism as a valuable clinical marker.