Polymorphisms in MMP-9 and TIMP-2 in Chinese Patients with Varicose Veins

Background Varicose veins (VVs), a common vascular disease, are functionally characterized by dilation and tortuosity and are widely prevalent in the adult population. The pathophysiology and molecular mechanism of VVs are still unclear. A genetic risk for VVs has been demonstrated, although no gene...

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Published in:The Journal of surgical research 2011-06, Vol.168 (1), p.e143-e148
Main Authors: Xu, Hong-mei, Ph.D, Zhao, Yun, B.A, Zhang, Xiang-man, M.A, Zhu, Ting, Ph.D, Fu, Wei-guo, M.D
Format: Article
Language:English
Subjects:
Aged
Case-Control Studies
China - epidemiology
Female
Genetic Predisposition to Disease - ethnology
Genetic Predisposition to Disease - genetics
Genotype
Humans
Male
Matrix Metalloproteinase 9 - genetics
Middle Aged
MMP-9
Polymorphism, Genetic - genetics
promoter polymorphism
Promoter Regions, Genetic - genetics
Risk Factors
Surgery
TIMP-2
Tissue Inhibitor of Metalloproteinase-2 - genetics
varicose vein
Varicose Veins - epidemiology
Varicose Veins - ethnology
Varicose Veins - genetics
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Summary:Background Varicose veins (VVs), a common vascular disease, are functionally characterized by dilation and tortuosity and are widely prevalent in the adult population. The pathophysiology and molecular mechanism of VVs are still unclear. A genetic risk for VVs has been demonstrated, although no genetic variant pertaining to VVs has been identified. Matrix metalloproteinases (MMPs) and their endogenous tissue inhibitors (TIMPs), which can prevent excessive extracellular matrix (ECM) degradation, greatly impact vascular remodeling and may play a vital role in patients with VVs. We evaluated a potential association between polymorphisms in the promoters of MMP-9 and TIMP-2 and the risk for VVs in the Chinese population. Materials and Methods Genotyping of the promoter region polymorphisms -1562C/T in MMP-9 and -418G/C in TIMP-2 was performed with PCR and restriction fragment length polymorphism (PCR-RFLP) assays with a group of 60 patients with VVs and 60 healthy controls. Purified PCR products were sequenced. Results A significant correlation was found between patients with VVs and controls at -1562C/T in MMP-9. The TIMP-2 gene polymorphism -418G/C was also associated with VVs. Conclusions Our results suggest that polymorphisms in the promoter region of MMP-9 and TIMP-2 are associated with VVs in the Chinese population
ISSN:0022-4804
1095-8673
DOI:10.1016/j.jss.2010.11.002