The activities of purine-catabolizing enzymes and the level of nitric oxide in rat kidneys subjected to methotrexate: protective effect of caffeic acid phenethyl ester

The aim of this experimental study was to investigate the possible role of nitric oxide (NO) levels, and activities of adenosine deaminase (ADA) and xanthine oxidase (XO) in the pathogenesis of methotrexate-induced nephrotoxicity, and was the effect of caffeic acid phenethyl ester (CAPE), the potent...

Full description

Saved in:
Bibliographic Details
Published in:Molecular and cellular biochemistry 2005-09, Vol.277 (1-2), p.165-170
Main Authors: Uz, Efkan, Oktem, Faruk, Yilmaz, H Ramazan, Uzar, Ertuğrul, Ozgüner, Fehmi
Format: Article
Language:English
Subjects:
Adenosine Deaminase - metabolism
Animal tissues
Animals
Antimetabolites, Antineoplastic - toxicity
Caffeic Acids - pharmacology
Folic Acid Antagonists - toxicity
Free Radical Scavengers - pharmacology
Free radicals
Kidney - drug effects
Kidney - metabolism
Male
Methotrexate - antagonists & inhibitors
Methotrexate - toxicity
Nitric oxide
Nitric Oxide - metabolism
Pathogenesis
Phenylethyl Alcohol - analogs & derivatives
Phenylethyl Alcohol - pharmacology
Purines - metabolism
Rats
Rats, Wistar
Rodents
Side effects
Xanthine Oxidase - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The aim of this experimental study was to investigate the possible role of nitric oxide (NO) levels, and activities of adenosine deaminase (ADA) and xanthine oxidase (XO) in the pathogenesis of methotrexate-induced nephrotoxicity, and was the effect of caffeic acid phenethyl ester (CAPE), the potent free radical scavenger, in decreasing the toxicity. A total of 19 adult male rats were divided into three experimental groups, as follows: control group, MTX-treated group, and MTX+CAPE treated group. MTX were administered intraperitoneally (i.p.) with 20 mg/kg for single dose. CAPE was administered i.p. with a dose of 10 micromol//kg once daily for 7 days. The injection of MTX induced a significant increase in the activities of ADA and XO, and NO levels in renal tissue of rats (p < 0.0001). Co-treatment with CAPE caused a significantly decrease activities of ADA and XO, and the levels of NO in renal tissue (p < 0.0001). The results of this study revealed that NO, XO and ADA may play an important role in the pathogenesis of MTX-induced oxidative renal damage. CAPE may have protective potential in this process and it will become a promising drug in the prevention of this undesired side effect of MTX.
ISSN:0300-8177
1573-4919
DOI:10.1007/s11010-005-5875-x