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UreA, the major urea/H + symporter in Aspergillus nidulans
We report here the characterization of UreA, a high-affinity urea/H + symporter of Aspergillus nidulans. The deletion of the encoding gene abolishes urea transport at low substrate concentrations, suggesting that in these conditions UreA is the sole transport system specific for urea in A. nidulans....
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| Published in: | Fungal genetics and biology 2010-12, Vol.47 (12), p.1023-1033 |
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| Main Authors: | , , , |
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| Language: | English |
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| container_end_page | 1033 |
| container_issue | 12 |
| container_start_page | 1023 |
| container_title | Fungal genetics and biology |
| container_volume | 47 |
| creator | Abreu, Cecilia Sanguinetti, Manuel Amillis, Sotiris Ramon, Ana |
| description | We report here the characterization of UreA, a high-affinity urea/H
+ symporter of
Aspergillus nidulans. The deletion of the encoding gene abolishes urea transport at low substrate concentrations, suggesting that in these conditions UreA is the sole transport system specific for urea in
A. nidulans. The
ureA gene is not inducible by urea or its precursors, but responds to nitrogen metabolite repression, necessitating for its expression the AreA GATA factor. In contrast to what was observed for other transporters in
A. nidulans, repression by ammonium is also operative during the isotropic growth phase. The activity of UreA is down-regulated post-translationally by ammonium-promoted endocytosis. A number of homologues of UreA have been identified in
A. nidulans and other
Aspergilli, which cluster in four groups, two of which contain the urea transporters characterized so far in fungi and plants. This phylogeny may have arisen by gene duplication events, giving place to putative transport proteins that could have acquired novel, still unidentified functions. |
| doi_str_mv | 10.1016/j.fgb.2010.07.004 |
| format | article |
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+ symporter of
Aspergillus nidulans. The deletion of the encoding gene abolishes urea transport at low substrate concentrations, suggesting that in these conditions UreA is the sole transport system specific for urea in
A. nidulans. The
ureA gene is not inducible by urea or its precursors, but responds to nitrogen metabolite repression, necessitating for its expression the AreA GATA factor. In contrast to what was observed for other transporters in
A. nidulans, repression by ammonium is also operative during the isotropic growth phase. The activity of UreA is down-regulated post-translationally by ammonium-promoted endocytosis. A number of homologues of UreA have been identified in
A. nidulans and other
Aspergilli, which cluster in four groups, two of which contain the urea transporters characterized so far in fungi and plants. This phylogeny may have arisen by gene duplication events, giving place to putative transport proteins that could have acquired novel, still unidentified functions.</description><identifier>ISSN: 1087-1845</identifier><identifier>EISSN: 1096-0937</identifier><identifier>DOI: 10.1016/j.fgb.2010.07.004</identifier><identifier>PMID: 20633690</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Aspergillus nidulans ; Aspergillus nidulans - classification ; Aspergillus nidulans - genetics ; Aspergillus nidulans - metabolism ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Gene Expression Regulation, Fungal ; Membrane Transport Proteins - genetics ; Membrane Transport Proteins - metabolism ; Molecular Sequence Data ; Phylogeny ; Quaternary Ammonium Compounds - metabolism ; UreA ; Urea - metabolism ; Urea transport ; Urea Transporters ; Urea/H + symporter</subject><ispartof>Fungal genetics and biology, 2010-12, Vol.47 (12), p.1023-1033</ispartof><rights>2010 Elsevier Inc.</rights><rights>Copyright © 2010 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-a25f8b94cbb7e02701d24fcdf456cb031a3d198ede2dee011720e06e8cd6d10a3</citedby><cites>FETCH-LOGICAL-c384t-a25f8b94cbb7e02701d24fcdf456cb031a3d198ede2dee011720e06e8cd6d10a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20633690$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abreu, Cecilia</creatorcontrib><creatorcontrib>Sanguinetti, Manuel</creatorcontrib><creatorcontrib>Amillis, Sotiris</creatorcontrib><creatorcontrib>Ramon, Ana</creatorcontrib><title>UreA, the major urea/H + symporter in Aspergillus nidulans</title><title>Fungal genetics and biology</title><addtitle>Fungal Genet Biol</addtitle><description>We report here the characterization of UreA, a high-affinity urea/H
+ symporter of
Aspergillus nidulans. The deletion of the encoding gene abolishes urea transport at low substrate concentrations, suggesting that in these conditions UreA is the sole transport system specific for urea in
A. nidulans. The
ureA gene is not inducible by urea or its precursors, but responds to nitrogen metabolite repression, necessitating for its expression the AreA GATA factor. In contrast to what was observed for other transporters in
A. nidulans, repression by ammonium is also operative during the isotropic growth phase. The activity of UreA is down-regulated post-translationally by ammonium-promoted endocytosis. A number of homologues of UreA have been identified in
A. nidulans and other
Aspergilli, which cluster in four groups, two of which contain the urea transporters characterized so far in fungi and plants. This phylogeny may have arisen by gene duplication events, giving place to putative transport proteins that could have acquired novel, still unidentified functions.</description><subject>Aspergillus nidulans</subject><subject>Aspergillus nidulans - classification</subject><subject>Aspergillus nidulans - genetics</subject><subject>Aspergillus nidulans - metabolism</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Membrane Transport Proteins - genetics</subject><subject>Membrane Transport Proteins - metabolism</subject><subject>Molecular Sequence Data</subject><subject>Phylogeny</subject><subject>Quaternary Ammonium Compounds - metabolism</subject><subject>UreA</subject><subject>Urea - metabolism</subject><subject>Urea transport</subject><subject>Urea Transporters</subject><subject>Urea/H + symporter</subject><issn>1087-1845</issn><issn>1096-0937</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqFkE1LAzEQhoMotlZ_gBfZmwfddrJfSfRUilqh4MWew24yW1P2y2RX6L83pdWjnmYGnvdleAi5pjClQLPZdlpuimkE_gY2BUhOyJiCyEIQMTvd75yFlCfpiFw4twWgNE3oORlFkMVxJmBMHtYW5_dB_4FBnW9bGwwW89kyuAvcru5a26MNTBPMXYd2Y6pqcEFj9FDljbskZ2VeObw6zglZPz-9L5bh6u3ldTFfhSrmSR_mUVryQiSqKBhCxIDqKCmVLpM0UwXENI81FRw1RhrRv8giQMiQK51pCnk8IbeH3s62nwO6XtbGKaz8D9gOTvIsESlwzv4lmRBMMC64J-mBVLZ1zmIpO2vq3O4kBbl3K7fSu5V7txKY9G595ubYPhQ16t_Ej0wPPB4A9Da-DFrplMFGoTYWVS91a_6o_wYqGogu</recordid><startdate>20101201</startdate><enddate>20101201</enddate><creator>Abreu, Cecilia</creator><creator>Sanguinetti, Manuel</creator><creator>Amillis, Sotiris</creator><creator>Ramon, Ana</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20101201</creationdate><title>UreA, the major urea/H + symporter in Aspergillus nidulans</title><author>Abreu, Cecilia ; Sanguinetti, Manuel ; Amillis, Sotiris ; Ramon, Ana</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-a25f8b94cbb7e02701d24fcdf456cb031a3d198ede2dee011720e06e8cd6d10a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Aspergillus nidulans</topic><topic>Aspergillus nidulans - classification</topic><topic>Aspergillus nidulans - genetics</topic><topic>Aspergillus nidulans - metabolism</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>Gene Expression Regulation, Fungal</topic><topic>Membrane Transport Proteins - genetics</topic><topic>Membrane Transport Proteins - metabolism</topic><topic>Molecular Sequence Data</topic><topic>Phylogeny</topic><topic>Quaternary Ammonium Compounds - metabolism</topic><topic>UreA</topic><topic>Urea - metabolism</topic><topic>Urea transport</topic><topic>Urea Transporters</topic><topic>Urea/H + symporter</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abreu, Cecilia</creatorcontrib><creatorcontrib>Sanguinetti, Manuel</creatorcontrib><creatorcontrib>Amillis, Sotiris</creatorcontrib><creatorcontrib>Ramon, Ana</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Fungal genetics and biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abreu, Cecilia</au><au>Sanguinetti, Manuel</au><au>Amillis, Sotiris</au><au>Ramon, Ana</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>UreA, the major urea/H + symporter in Aspergillus nidulans</atitle><jtitle>Fungal genetics and biology</jtitle><addtitle>Fungal Genet Biol</addtitle><date>2010-12-01</date><risdate>2010</risdate><volume>47</volume><issue>12</issue><spage>1023</spage><epage>1033</epage><pages>1023-1033</pages><issn>1087-1845</issn><eissn>1096-0937</eissn><abstract>We report here the characterization of UreA, a high-affinity urea/H
+ symporter of
Aspergillus nidulans. The deletion of the encoding gene abolishes urea transport at low substrate concentrations, suggesting that in these conditions UreA is the sole transport system specific for urea in
A. nidulans. The
ureA gene is not inducible by urea or its precursors, but responds to nitrogen metabolite repression, necessitating for its expression the AreA GATA factor. In contrast to what was observed for other transporters in
A. nidulans, repression by ammonium is also operative during the isotropic growth phase. The activity of UreA is down-regulated post-translationally by ammonium-promoted endocytosis. A number of homologues of UreA have been identified in
A. nidulans and other
Aspergilli, which cluster in four groups, two of which contain the urea transporters characterized so far in fungi and plants. This phylogeny may have arisen by gene duplication events, giving place to putative transport proteins that could have acquired novel, still unidentified functions.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>20633690</pmid><doi>10.1016/j.fgb.2010.07.004</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1087-1845 |
| ispartof | Fungal genetics and biology, 2010-12, Vol.47 (12), p.1023-1033 |
| issn | 1087-1845 1096-0937 |
| language | eng |
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| source | ScienceDirect Freedom Collection |
| subjects | Aspergillus nidulans Aspergillus nidulans - classification Aspergillus nidulans - genetics Aspergillus nidulans - metabolism Fungal Proteins - genetics Fungal Proteins - metabolism Gene Expression Regulation, Fungal Membrane Transport Proteins - genetics Membrane Transport Proteins - metabolism Molecular Sequence Data Phylogeny Quaternary Ammonium Compounds - metabolism UreA Urea - metabolism Urea transport Urea Transporters Urea/H + symporter |
| title | UreA, the major urea/H + symporter in Aspergillus nidulans |
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