Altered microRNAs in STHdhQ111/HdhQ111 cells: miR-146a targets TBP

We studied expression of 90 miRNAs in STHdhQ111/HdhQ111 cells, a model for Huntington’s disease and compared with that obtained in STHdhQ7/HdhQ7 cells. Fifteen miRNAs were down regulated and 12 miRNAs were up regulated more than 2-fold. Such changes were statistically significant. One hundred and fo...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2010-06, Vol.396 (3), p.742-747
Main Authors: Sinha, Mithun, Ghose, Jayeeta, Das, Eashita, Bhattarcharyya, Nitai P.
Format: Article
Language:English
Subjects:
MicroRNA-146a
STHdhQ111/HdhQ111 cells
Tata Binding Protein
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Summary:We studied expression of 90 miRNAs in STHdhQ111/HdhQ111 cells, a model for Huntington’s disease and compared with that obtained in STHdhQ7/HdhQ7 cells. Fifteen miRNAs were down regulated and 12 miRNAs were up regulated more than 2-fold. Such changes were statistically significant. One hundred and forty-two genes are experimentally known targets of these altered miRNAs. It has been predicted that miR-146a may target Tata Binding Protein (TBP). Using luciferase reporter assays with 3′-UTRs of TBP, over-expression and inhibition of miR-146a, we showed that miR-146a targets TBP. Regulation of TBP by miR-146a may contribute to HD pathogenesis.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2010.05.007