Transgenic tobacco and peanut plants expressing a mustard defensin show resistance to fungal pathogens

Defensins are small positively charged, antimicrobial peptides (~5 kDa in size) and some of them exhibit potent antifungal activity. We have cloned the complete cDNA containing an ORF of 243 bp of a defensin of mustard. The deduced amino acid sequence of the peptide showed more than 90% identity to...

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Bibliographic Details
Published in:Plant cell reports 2008-11, Vol.27 (11), p.1777-1786
Main Authors: Swathi Anuradha, T, Divya, K, Jami, S. K, Kirti, P. B
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Arachis - genetics
Arachis - immunology
Arachis - microbiology
Arachis hypogaea
Base Sequence
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Biotic and Abiotic Stress
Blotting, Southern
Cell Biology
Cercospora arachidicola
Defensins - chemistry
Defensins - genetics
Disease resistance
DNA Primers
Fundamental and applied biological sciences. Psychology
Fusarium - pathogenicity
Fusarium moniliforme
Life Sciences
Molecular Sequence Data
Nicotiana - genetics
Nicotiana - immunology
Nicotiana - microbiology
Pathogens
Peanuts
Peptides
Phytophthora - pathogenicity
Phytophthora parasitica
Plant Biochemistry
Plant Sciences
Plants, Genetically Modified - genetics
Plants, Genetically Modified - immunology
Plants, Genetically Modified - microbiology
Raphanus sativus
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
Tobacco
Transgenic plants
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Summary:Defensins are small positively charged, antimicrobial peptides (~5 kDa in size) and some of them exhibit potent antifungal activity. We have cloned the complete cDNA containing an ORF of 243 bp of a defensin of mustard. The deduced amino acid sequence of the peptide showed more than 90% identity to the amino acid sequence of the well-characterized defensins, RsAFP-1 and RsAFP-2 of Raphanus sativus. We have generated and characterized transgenic tobacco and peanut plants constitutively expressing the mustard defensin. Transgenic tobacco plants were resistant to the fungal pathogens, Fusarium moniliforme and Phytophthora parasitica pv. nicotianae. Transgenic peanut plants showed enhanced resistance against the pathogens, Pheaoisariopsis personata and Cercospora arachidicola, which jointly cause serious late leaf spot disease. These observations indicate that the mustard defensin gene can be deployed for deriving fungal disease resistance in transgenic crops.
ISSN:0721-7714
1432-203X
DOI:10.1007/s00299-008-0596-8