Deletion of a prophage-like element causes attenuation of Salmonella enterica serovar Enteritidis and promotes protective immunity

• Published in: Vaccine 2010-07, Vol.28 (33), p.5458-5466
• Main Authors: Araya, Daniela V, Quiroz, Tania S, Tobar, Hugo E, Lizana, Rodrigo J, Quezada, Carolina P, Santiviago, Carlos A, Riedel, Claudia A, Kalergis, Alexis M, Bueno, Susan M
• Format: Article
• Language: English
• Subjects: Allergy and Immunology; Animals; Antibodies, Bacterial - immunology; Applied microbiology; Bacterial immunity; Bacteriology; Base Sequence; Biological and medical sciences; Eggs; Foodborne diseases; Fundamental and applied biological sciences. Psychology; Genes; Immunoglobulin G - immunology; Infections; Mice; Microbiology; Miscellaneous; Pathogens; Phagocytes - immunology; Phagocytes - microbiology; Poultry; Prophage; Prophages - genetics; Prophages - immunology; Salmonella; Salmonella enterica; Salmonella enterica serovar Enteritidis; Salmonella enteritidis - genetics; Salmonella enteritidis - immunology; Salmonella enteritidis - pathogenicity; Salmonella Infections - genetics; Salmonella Infections - immunology; Salmonella Infections - prevention & control; Salmonella typhimurium; Salmonella Vaccines - genetics; Salmonella Vaccines - immunology; Sequence Deletion - genetics; Sequence Deletion - immunology; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Vaccines, Attenuated - genetics; Vaccines, Attenuated - immunology; Virulence Factors - genetics; Virulence Factors - immunology
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2010.05.073

Abstract Salmonella enterica serovar Enteritidis ( S. Enteritidis) is a wide host range serovar belonging to the S. enterica genus. Worldwide, it is one of the most frequent causes of food borne disease. Similar to S. Typhimurium, some virulence genes of S. Enteritidis are located in pathogenicity islands and prophages. In this study we have generated a mutant strain of S. Enteritidis lacking a prophage-like element, denominated SE12. The resulting mutant strain was attenuated and promoted protective immunity in infected mice. Although S. Enteritidis strains lacking the complete prophage SE12 remained capable of surviving inside phagocytic cells, they showed a significantly reduced capacity to colonize internal organs and failed to cause lethal disease in mice. Consistent with these data, infection with S . Enteritidis strains lacking prophage SE12 promoted the production of anti- Salmonella IgG antibodies and led to protection against a challenge with virulent strains of S . Enteritidis. These results suggest that strains lacking this prophage can induce a protective immunity in mice and be considered as potential attenuated vaccines against S. Enteritidis.