Proteomic analysis of rat brains in a model of neuropathic pain following exposure to electroconvulsive stimulation

Some reports have shown that electroconvulsive shock therapy is effective for treating refractory neuropathic pain. However, its mechanism of action remains unknown. This study analyzes changes in protein expression in the brainstems of neuropathic pain model rats with or without electroconvulsive s...

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Bibliographic Details
Published in:Biomedical Research 2011, Vol.32(2), pp.91-102
Main Authors: Kamagata, Chihiro, Tsuboko, Yosiaki, Okabe, Tadashi, Sato, Chiyo, Sakamoto, Atsuhiro
Format: Article
Language:English
Subjects:
Animals
Brain Stem - metabolism
Brain Stem - physiopathology
Disease Models, Animal
Electroconvulsive Therapy
Electrophoresis, Gel, Two-Dimensional
Gene Expression
Hyperalgesia - genetics
Hyperalgesia - metabolism
Male
Mass Spectrometry
Neuralgia - genetics
Neuralgia - metabolism
Neuralgia - physiopathology
Neuralgia - therapy
Pain Threshold - physiology
Proteins - genetics
Proteins - metabolism
Proteomics
Rats
Rats, Sprague-Dawley
Reaction Time - physiology
Sciatic Nerve - injuries
Sciatic Nerve - metabolism
Sciatic Nerve - physiopathology
Sciatic Neuropathy - genetics
Sciatic Neuropathy - metabolism
Sciatic Neuropathy - physiopathology
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Summary:Some reports have shown that electroconvulsive shock therapy is effective for treating refractory neuropathic pain. However, its mechanism of action remains unknown. This study analyzes changes in protein expression in the brainstems of neuropathic pain model rats with or without electroconvulsive stimulation (ECS). A neuropathic pain model rat is produced by chronic constrictive injury (CCI) of the sciatic nerve. An ECS was administered to rodents once daily for 6 days after the CCI operation. After ECS, the latency to withdrawal from thermal stimulation was significantly increased. The expression of several proteins was changed after CCI. Ten proteins that increased after CCI then had decreased expression levels (close to control) after ECS, and 8 proteins that decreased after CCI then had increased expression levels (close to control) after ECS. In conclusion, ECS improved thermal hypersensitivity in a rat CCI model. Proteomic analysis showed that altered expression levels of proteins in the brainstem of CCI model rats returned to close to control levels after ECS, including many proteins associated with pain. This trend suggests an association of ECS with improved hypersensitivity, and these results may help elucidate the mechanism of this effect.
ISSN:0388-6107
1880-313X
DOI:10.2220/biomedres.32.91