Tracking Bismuth Antiulcer Drug Uptake in Single Helicobacter pylori Cells

Bismuth-based drugs have long been used for the treatment of Helicobacter pylori infection. In this work, the metal content in H. pylori was monitored at the single-cell level by time-resolved inductively coupled plasma mass spectrometry, and ∼2.9 × 107 Mg atoms/cell was determined for the wild-type...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2011-05, Vol.133 (19), p.7355-7357
Main Authors: Tsang, Cheuk-Nam, Ho, Koon-Sing, Sun, Hongzhe, Chan, Wing-Tat
Format: Article
Language:English
Subjects:
Anti-Ulcer Agents - pharmacokinetics
Biological Transport
Bismuth - pharmacokinetics
Helicobacter pylori - metabolism
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Summary:Bismuth-based drugs have long been used for the treatment of Helicobacter pylori infection. In this work, the metal content in H. pylori was monitored at the single-cell level by time-resolved inductively coupled plasma mass spectrometry, and ∼2.9 × 107 Mg atoms/cell was determined for the wild-type. Bacteria treated with a Bi antiulcer drug deposited nearly 1.0 × 106 Bi atoms/cell, whereas the uptake process took ∼3 h to reach the half-maximum. Interference of ferric ions on bismuth uptake was demonstrated, suggesting that the metallodrug can utilize certain iron-transport pathways in the pathogen. The approach provides a general strategy for monitoring metals in single cells, facilitating exploration of metal-relevant bioprocesses.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja2013278