Isolation of 21 polymorphic microsatellite markers for the Virginia opossum (Didelphis virginiana)

Twenty-one polymorphic microsatellite markers were developed for the Virginia opossum (Didelphis virginiana). The number of alleles ranged from two to 13 and observed heterozygosities ranged from 0.464 to 0.964. Significant heterozygote deficiencies were observed at three loci and null alleles were...

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Bibliographic Details
Published in:Molecular ecology resources 2009-07, Vol.9 (4), p.1200-1202
Main Authors: FIKE, JENNIFER A, BEASLEY, JAMES C, RHODES, JR, OLIN E
Format: Article
Language:English
Subjects:
Didelphis virginiana
marsupial
Marsupialia
microsatellite
microsatellite repeats
Virginia opossum
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Summary:Twenty-one polymorphic microsatellite markers were developed for the Virginia opossum (Didelphis virginiana). The number of alleles ranged from two to 13 and observed heterozygosities ranged from 0.464 to 0.964. Significant heterozygote deficiencies were observed at three loci and null alleles were detected at five loci. Evidence for gametic disequilibrium was observed between three sets of paired loci after a sequential Bonferroni correction was applied. These markers will enable us to investigate the mating tactics, movement behaviour and social structure of Virginia opossum populations inhabiting fragmented agricultural landscapes.
ISSN:1755-0998
1755-098X
1755-0998
DOI:10.1111/j.1755-0998.2009.02606.x