Eight novel tetranucleotide and five cross-species dinucleotide microsatellite loci for the ornate chorus frog (Pseudacris ornata)

We describe the cloning and characterization of eight novel tetranucleotide microsatellite loci in the ornate chorus frog (Pseudacris ornata). We also screened 26 loci from GenBank that were isolated from other Pseudacris species and obtained consistent product from five of these dinucleotide loci....

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Bibliographic Details
Published in:Molecular ecology resources 2009-03, Vol.9 (2), p.622-624
Main Authors: DEGNER, JACOB F, HETHER, TYLER D, HOFFMAN, ERIC A
Format: Article
Language:English
Subjects:
Anura
biotin enrichment
chorus frog
microsatellite
microsatellite repeats
Pseudacris
Pseudacris ornata
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Summary:We describe the cloning and characterization of eight novel tetranucleotide microsatellite loci in the ornate chorus frog (Pseudacris ornata). We also screened 26 loci from GenBank that were isolated from other Pseudacris species and obtained consistent product from five of these dinucleotide loci. All loci are polymorphic. In our sample of 26 frogs from a natural population, polymorphism ranged from 1 to 22 alleles per locus with expected heterozygosities ranging from 0 to 0.958. These loci enable high-resolution studies of P. ornata. Moreover, cross-species amplification success suggests they will also be useful for other chorus frog species.
ISSN:1755-0998
1755-098X
1755-0998
DOI:10.1111/j.1755-0998.2008.02478.x