A new approach to the analysis of nicarbazin and ionophores in eggs by HPLC/MS/MS

An HPLC/MS/MS method has been developed and validated for the quantification and confirmation of nicarbazin and ionophores (lasalocid, monensin, salinomycin, and narasin) in eggs. Nicarbazin is determined in the negative electrospray mode with a basic mobile phase that supports creation of negative...

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Bibliographic Details
Published in:Journal of AOAC International 2011-03, Vol.94 (2), p.428-435
Main Authors: Dmitrovic, Jasna, Durden, David A
Format: Article
Language:English
Subjects:
Animals
Chickens
Chromatography, High Pressure Liquid - methods
Coccidiostats - chemistry
Eggs
Eggs - analysis
High performance liquid chromatography
Ionophores
Ionophores - chemistry
Molecular Structure
Nicarbazin - chemistry
Tandem Mass Spectrometry - methods
Veterinary drugs
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Summary:An HPLC/MS/MS method has been developed and validated for the quantification and confirmation of nicarbazin and ionophores (lasalocid, monensin, salinomycin, and narasin) in eggs. Nicarbazin is determined in the negative electrospray mode with a basic mobile phase that supports creation of negative ions. Consequently, our ability to maintain instrument sensitivity over time has significantly improved. The analysis of the ionophores is done in the positive electrospray mode using ammonium buffer for HPLC separation. Monitoring ammonium adduct parent ions resulted in enhanced sensitivity and better reproducibility of the ionophore analysis. The validation of this improved HPLC/MS/MS method for the detection of nicarbazin and the ionophores demonstrated excellent precision of below 10% RSD and lower LOD values (microg/kg) for nicarbazin (0.018), lasalocid (0.015), monensin (0.015), salinomycin (0.033), and narasin (0.039).
ISSN:1060-3271
1944-7922
DOI:10.1093/jaoac/94.2.428