Microsatellite markers for the Amazon peacock bass (Cichla piquiti)

A set of primers to amplify 10 microsatellite DNA loci was developed for the Neotropical fish Cichla piquiti, one of the largest sized cichlids in the Amazon Basin. These loci were used to genotype individuals from two populations, one native population from the Tocantins River, the other an introdu...

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Bibliographic Details
Published in:Molecular ecology resources 2009, Vol.9 (1), p.239-241
Main Authors: CARVALHO, D.C, OLIVEIRA, D.A.A, SAMPAIO, I, BEHEREGARAY, L.B
Format: Article
Language:English
Subjects:
Cichla
Cichlidae
conservation genetics
Freshwater
introduced populations
microsatellite repeats
microsatellites
Peacock bass
Tucunaré
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Summary:A set of primers to amplify 10 microsatellite DNA loci was developed for the Neotropical fish Cichla piquiti, one of the largest sized cichlids in the Amazon Basin. These loci were used to genotype individuals from two populations, one native population from the Tocantins River, the other an introduced population in southeast Brazil, Upper Paraná River. Cross-amplification was also successful for another species of peacock bass, C. kelberi. An average of 4.4 alleles per locus (2-9 alleles) was detected. These markers will be useful for the characterization of genetic structure of native populations, and also for invasive biology studies since Cichla species have been introduced in many river basins outside their native ranges.
ISSN:1755-0998
1755-098X
1755-0998
DOI:10.1111/j.1755-0998.2008.02425.x