Specific and Rapid Quantification of Viable Listeria monocytogenes Using Fluorescence in situ Hybridization in Combination with Filter Cultivation

We developed a new method that rapidly and specifically enumerates only viable Listeria monocytogenes in food using fluorescence in situ hybridization in combination with filter cultivation (FISHFC). Viable L. monocytogenes could be specifically quantified within 16 h using an Alexa647-labeled mRL-2...

Full description

Saved in:
Bibliographic Details
Published in:Microbes and Environments 2009, Vol.24(3), pp.273-275
Main Authors: Fuchizawa, Ikufumi, Shimizu, Shigemasa, Ootsubo, Masashi, Kawai, Yuji, Yamazaki, Koji
Format: Article
Language:English
Subjects:
FISHFC
fluorescence in situ hybridization
Listeria monocytogenes
specific detection
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We developed a new method that rapidly and specifically enumerates only viable Listeria monocytogenes in food using fluorescence in situ hybridization in combination with filter cultivation (FISHFC). Viable L. monocytogenes could be specifically quantified within 16 h using an Alexa647-labeled mRL-2 probe. The coefficient of the correlation between the new method and the conventional plating method was 0.959.
ISSN:1342-6311
1347-4405
DOI:10.1264/jsme2.ME09102