Characterization of polymorphic microsatellite markers in the Dalmatian wall lizard Podarcis melisellensis (Squamata: Lacertidae)

We describe polymerase chain reaction primers and amplification conditions for 13 highly polymorphic microsatellite DNA loci isolated from the Dalmatian wall lizard, Podarcis melisellensis. The number of alleles per locus ranged from 12 to 41, with levels of observed heterozygosity between 0.62 and...

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Bibliographic Details
Published in:Molecular ecology resources 2009, Vol.9 (1), p.299-301
Main Authors: HUYGHE, K, BREUGELMANS, K, SMALL, M, TADIĆ, Z, VAN DAMME, R, VANHOOYDONCK, B, BACKELJAU, T
Format: Article
Language:English
Subjects:
cross-species amplification
Lacertidae
Lacertilia
microsatellite repeats
microsatellites
Podarcis melisellensis
Podarcis sicula
Squamata
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Summary:We describe polymerase chain reaction primers and amplification conditions for 13 highly polymorphic microsatellite DNA loci isolated from the Dalmatian wall lizard, Podarcis melisellensis. The number of alleles per locus ranged from 12 to 41, with levels of observed heterozygosity between 0.62 and 0.94. Most of these loci were successfully cross-amplified in the closely related species P. sicula, but levels of polymorphism were always lower.
ISSN:1755-0998
1755-098X
1755-0998
DOI:10.1111/j.1755-0998.2008.02381.x