Oxidation of glyceraldehyde-3-phosphate dehydrogenase decreases sperm motility

The relation between the activity of the sperm-specific glyceraldehyde-3-phosphate dehydrogenase (GAPDS) and the motility of sperms was investigated. It was found that the mean value of GAPDS activity in sperm samples with low motility is 2.5-3-fold lower than that in samples with high motility. Spe...

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Bibliographic Details
Published in:Biochemistry (Moscow) 2011-02, Vol.76 (2), p.268-272
Main Authors: Elkina, Yu. L, Atroshchenko, M. M, Bragina, E. E, Muronetz, V. I, Schmalhausen, E. V
Format: Article
Language:English
Subjects:
Animals
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Cellular biology
Chemical properties
Dehydrogenase
Enzymes
Fertility
Glyceraldehyde-3-Phosphate Dehydrogenases - deficiency
Glyceraldehyde-3-Phosphate Dehydrogenases - physiology
Glycolysis - drug effects
Horses
Humans
Hydrogen peroxide
Hydrogen Peroxide - pharmacology
Hydroxyl radicals
Life Sciences
Male
Men
Microbiology
Monosaccharides
Oxidation
Oxidation-Reduction
Oxidizing agents
Phosphates
Reactive Oxygen Species - metabolism
Sperm Motility - drug effects
Sperm Motility - physiology
Spermatozoa
Spermatozoa - enzymology
Sugars
Superoxide
Superoxides - metabolism
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Summary:The relation between the activity of the sperm-specific glyceraldehyde-3-phosphate dehydrogenase (GAPDS) and the motility of sperms was investigated. It was found that the mean value of GAPDS activity in sperm samples with low motility is 2.5-3-fold lower than that in samples with high motility. Sperm motility was shown to diminish in the presence of superoxide anion, hydroxyl radical, and hydrogen peroxide. The decrease in sperm motility in the presence of hydrogen peroxide was proportional to the concentration of the oxidant and correlated with the decrease in GAPDS activity (r = 0.96). Based on the literature data on the importance of GAPDS for the motility of sperms together with the presented observations, it was concluded that the decrease in the sperm motility in the presence of reactive oxygen species is due to the oxidation of GAPDS and inhibition of glycolysis.
ISSN:0006-2979
1608-3040
DOI:10.1134/S0006297911020143