Role of Sox2 and Oct4 in predicting survival of hepatocellular carcinoma patients after hepatectomy

• Published in: Clinical biochemistry 2011-06, Vol.44 (8), p.582-589
• Main Authors: Huang, Pinzhu, Qiu, Jiliang, Li, Binkui, Hong, Jian, Lu, Canliang, Wang, Li, Wang, Jianping, Hu, Yezhu, Jia, Weihua, Yuan, Yunfei
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Carcinoma, Hepatocellular - genetics; Carcinoma, Hepatocellular - mortality; Carcinoma, Hepatocellular - surgery; Cell Line; Cell Line, Tumor; Disease-Free Survival; Female; Gastroenterology. Liver. Pancreas. Abdomen; Hep G2 Cells; Hepatectomy - mortality; Hepatocellular carcinoma; Humans; Investigative techniques, diagnostic techniques (general aspects); Liver Neoplasms - genetics; Liver Neoplasms - mortality; Liver Neoplasms - surgery; Liver, biliary tract, pancreas, portal circulation, spleen; Liver. Biliary tract. Portal circulation. Exocrine pancreas; Male; Medical sciences; Middle Aged; mRNA expression; Multivariate Analysis; Oct4; Octamer Transcription Factor-3 - genetics; Prognosis; Reverse Transcriptase Polymerase Chain Reaction; Sox2; SOXB1 Transcription Factors - genetics; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgery of the digestive system; Tumors
• ISSN: 0009-9120; 1873-2933
• DOI: 10.1016/j.clinbiochem.2011.02.012

The present study was aimed to explore the prognostic strength of Sox2 and Oct4A in hepatocellular carcinoma (HCC). We investigated the expression of Sox2 and Oct4A in five hepatoma cell lines, one immortalized normal liver cell line, HCC tissues with matched nontumorous liver tissues and normal liver tissues by reverse transcription-polymerase chain reaction. Sox2 and Oct4A mRNA were overexpressed in hepatoma cell lines and tumor tissues. Sox2 or Oct4A positive expression was significantly associated with an aggressive phenotype. Both univariate and multivariate analyses revealed that Sox2 or Oct4A was an independent prognostic factor for HCC. When using subgroup analysis, the patients with a co-expression of Sox2/Oct4A had the poorest prognosis. Further analysis demonstrated that Sox2 alone or Sox2/Oct4A could stratify outcome in HCC patients with early stage. Sox2 and Oct4A can be novel predictors of poor prognosis for patients undergoing resection of HCC.