Isolation and characterization of microsatellite markers in Oligoryzomys longicaudatus (Muridae, Sigmodontinae, Oryzomini), the natural reservoir of genotype Andes hantavirus

The rodent Oligoryzomys longicaudatus or long-tailed pygmy rice rat is the reservoir of the aetiological agent of the hantavirus pulmonary syndrome in southern Argentina and Chile. We characterize 11 polymorphic microsatellite loci which would be useful for studies on microgeographical population st...

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Bibliographic Details
Published in:Molecular ecology notes 2008-11, Vol.8 (6), p.1466-1468
Main Authors: GONZÁLEZ-ITTIG, RAÚL E, SALAZAR-BRAVO, JORGE, POLOP, JAIME J, GARDENAL, CRISTINA N
Format: Article
Language:English
Subjects:
Hantavirus
hantavirus pulmonary syndrome
microsatellite repeats
microsatellites
Muridae
Oligoryzomys longicaudatus
Oryza sativa
Sigmodontinae
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Summary:The rodent Oligoryzomys longicaudatus or long-tailed pygmy rice rat is the reservoir of the aetiological agent of the hantavirus pulmonary syndrome in southern Argentina and Chile. We characterize 11 polymorphic microsatellite loci which would be useful for studies on microgeographical population structure in the species. Amplification of these loci in 42 individuals from four natural populations revealed four to 21 alleles per locus, and values of observed heterozygosities ranging from 0.371 to 0.896. Cross-species amplifications showed that some of the primers designed may be useful for other species of the genus Oligoryzomys.
ISSN:1755-0998
1755-098X
1471-8278
1755-0998
1471-8286
DOI:10.1111/j.1755-0998.2008.02231.x