Characterization of microsatellite loci in the Hawaiian endemic shrub Schiedea adamantis (Caryophyllaceae) and amplification in related species and genera

Schiedea adamantis is a rare, perennial shrub endemic to the Hawaiian island of Oahu where it consists of a single population. Using a nonradioactive protocol, 12 microsatellite primers were developed that consisted of di-, tri-, penta- and hexanucleotide repeats. Using multiplexed reactions, all bu...

Full description

Saved in:
Bibliographic Details
Published in:Molecular ecology notes 2008-09, Vol.8 (5), p.1081-1084
Main Authors: CULLEY, T.M, WELLER, S.G, SAKAI, A.K, PUTNAM, K.A
Format: Article
Language:English
Subjects:
Caryophyllaceae
gynodioecy
Hawaii
Honckenya
Honckenya peploides
Schiedea
Schiedea adamantis
Silene
Silene lanceolata
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Schiedea adamantis is a rare, perennial shrub endemic to the Hawaiian island of Oahu where it consists of a single population. Using a nonradioactive protocol, 12 microsatellite primers were developed that consisted of di-, tri-, penta- and hexanucleotide repeats. Using multiplexed reactions, all but two primers exhibited polymorphism with an average of 3.67 alleles per primer. Most primers also amplified in 28 additional Schiedea species, revealing wide applicability across the genus; eight and nine primers also amplified in Honckenya peploides and Silene lanceolata, respectively, related genera in the Caryophyllaceae. This is the first known report of microsatellite primers developed in Schiedea.
ISSN:1755-0998
1755-098X
1471-8278
1755-0998
1471-8286
DOI:10.1111/j.1755-0998.2008.02161.x