Isolation and characterization of microsatellite loci for Florida largemouth bass, Micropterus salmoides floridanus, and other micropterids

We isolated and characterized 52 novel microsatellite markers from Florida largemouth bass, Micropterus salmoides floridanus, for use in conservation, management and population genetic studies. Markers were assessed in M. s. floridanus from peninsular Florida (n = 30) and averaged eight alleles per...

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Bibliographic Details
Published in:Molecular ecology notes 2008, Vol.8 (1), p.178-184
Main Authors: LUTZ-CARRILLO, DIJAR J, HAGEN, CRIS, DUECK, LUCY A, GLENN, TRAVIS C
Format: Article
Language:English
Subjects:
Centrarchidae
Florida largemouth bass
micropterids
Micropterus
microsatellite repeats
microsatellites
simple sequence repeats
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Summary:We isolated and characterized 52 novel microsatellite markers from Florida largemouth bass, Micropterus salmoides floridanus, for use in conservation, management and population genetic studies. Markers were assessed in M. s. floridanus from peninsular Florida (n = 30) and averaged eight alleles per locus with observed heterozygosity of 0.57 (range 0-0.97). Cross-taxa amplification was successful among 88% of tested congeners. These polymorphic and potentially taxon-diagnostic markers contribute to the limited number of microsatellites currently available for micropterids and specifically M. s. floridanus.
ISSN:1471-8278
1755-098X
1471-8286
1755-0998
DOI:10.1111/j.1471-8286.2007.01917.x