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Isolation and characterization of microsatellite loci for Florida largemouth bass, Micropterus salmoides floridanus, and other micropterids
We isolated and characterized 52 novel microsatellite markers from Florida largemouth bass, Micropterus salmoides floridanus, for use in conservation, management and population genetic studies. Markers were assessed in M. s. floridanus from peninsular Florida (n = 30) and averaged eight alleles per...
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Published in: | Molecular ecology notes 2008, Vol.8 (1), p.178-184 |
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description | We isolated and characterized 52 novel microsatellite markers from Florida largemouth bass, Micropterus salmoides floridanus, for use in conservation, management and population genetic studies. Markers were assessed in M. s. floridanus from peninsular Florida (n = 30) and averaged eight alleles per locus with observed heterozygosity of 0.57 (range 0-0.97). Cross-taxa amplification was successful among 88% of tested congeners. These polymorphic and potentially taxon-diagnostic markers contribute to the limited number of microsatellites currently available for micropterids and specifically M. s. floridanus. |
doi_str_mv | 10.1111/j.1471-8286.2007.01917.x |
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These polymorphic and potentially taxon-diagnostic markers contribute to the limited number of microsatellites currently available for micropterids and specifically M. s. floridanus.</description><subject>Centrarchidae</subject><subject>Florida largemouth bass</subject><subject>micropterids</subject><subject>Micropterus</subject><subject>microsatellite repeats</subject><subject>microsatellites</subject><subject>simple sequence repeats</subject><issn>1471-8278</issn><issn>1755-098X</issn><issn>1471-8286</issn><issn>1755-0998</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNqNkc1u1DAUhSMEoqXwCuAdGxL8k8TOggUatUOr_iC1VdlZN8l1x0MyntqJmPIKvDQOaWeNN76yv3Ou7rlJQhjNWDyf1xnLJUsVV2XGKZUZZRWT2e5Fcrj_eLmvpTpI3oSwppTJiuevkwPOClXIgh4mf06D62CwbkNg05JmBR6aAb39PT86Q3rbeBdgwK6zA5LONZYY58lJ57xtgXTg77F347AiNYTwiVxMgm00GQMJ0PXOthiImfHNGImplRtW6GfzibVteJu8MtAFfPd0HyU3J8c3i2_p-dXydPH1PG1yQWVaQiVKk6NoEbCOcwMrERTP6xpZIxhvmSwrVgoJ0PK6aLECYziXtC5yo8RR8nG23Xr3MGIYdG9DE6eDDboxaFVKyZWsaCTVTE4BBI9Gb73twT9qRvW0CL3WU8Z6yltPi9D_FqF3Ufr-qclY99juhc_JR-DLDPyyHT7-t7G-OL6cqqhPZ70NA-72evA_dSmFLPTd5VJ_X6jl9Y-zM80i_2HmDTgN994GfXvNKROUKiGKkou_x5izbg</recordid><startdate>2008</startdate><enddate>2008</enddate><creator>LUTZ-CARRILLO, DIJAR J</creator><creator>HAGEN, CRIS</creator><creator>DUECK, LUCY A</creator><creator>GLENN, TRAVIS C</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2008</creationdate><title>Isolation and characterization of microsatellite loci for Florida largemouth bass, Micropterus salmoides floridanus, and other micropterids</title><author>LUTZ-CARRILLO, DIJAR J ; HAGEN, CRIS ; DUECK, LUCY A ; GLENN, TRAVIS C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4307-6a936f4e3deaeb917a16ea824bbe1c312d17691637aad2b5de9aff2270b54f83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Centrarchidae</topic><topic>Florida largemouth bass</topic><topic>micropterids</topic><topic>Micropterus</topic><topic>microsatellite repeats</topic><topic>microsatellites</topic><topic>simple sequence repeats</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LUTZ-CARRILLO, DIJAR J</creatorcontrib><creatorcontrib>HAGEN, CRIS</creatorcontrib><creatorcontrib>DUECK, LUCY A</creatorcontrib><creatorcontrib>GLENN, TRAVIS C</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular ecology notes</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LUTZ-CARRILLO, DIJAR J</au><au>HAGEN, CRIS</au><au>DUECK, LUCY A</au><au>GLENN, TRAVIS C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterization of microsatellite loci for Florida largemouth bass, Micropterus salmoides floridanus, and other micropterids</atitle><jtitle>Molecular ecology notes</jtitle><addtitle>Mol Ecol Resour</addtitle><date>2008</date><risdate>2008</risdate><volume>8</volume><issue>1</issue><spage>178</spage><epage>184</epage><pages>178-184</pages><issn>1471-8278</issn><issn>1755-098X</issn><eissn>1471-8286</eissn><eissn>1755-0998</eissn><abstract>We isolated and characterized 52 novel microsatellite markers from Florida largemouth bass, Micropterus salmoides floridanus, for use in conservation, management and population genetic studies. Markers were assessed in M. s. floridanus from peninsular Florida (n = 30) and averaged eight alleles per locus with observed heterozygosity of 0.57 (range 0-0.97). Cross-taxa amplification was successful among 88% of tested congeners. These polymorphic and potentially taxon-diagnostic markers contribute to the limited number of microsatellites currently available for micropterids and specifically M. s. floridanus.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>21585750</pmid><doi>10.1111/j.1471-8286.2007.01917.x</doi><tpages>7</tpages></addata></record> |
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language | eng |
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source | Wiley-Blackwell Read & Publish Collection |
subjects | Centrarchidae Florida largemouth bass micropterids Micropterus microsatellite repeats microsatellites simple sequence repeats |
title | Isolation and characterization of microsatellite loci for Florida largemouth bass, Micropterus salmoides floridanus, and other micropterids |
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