Lentinus (Panus) tigrinus augmentation of a historically contaminated soil: Matrix decontamination and structure and function of the resident bacterial community

• Published in: Journal of hazardous materials 2011-02, Vol.186 (2-3), p.1263-1270
• Main Authors: Federici, E., Giubilei, M.A., Cajthaml, T., Petruccioli, M., D’Annibale, A.
• Format: Article
• Language: English
• Subjects: Applied sciences; Aromatic hydrocarbons; Bacteria; bacterial communities; Biodegradation, Environmental; Biodiversity; biphenyl; catechol; Catechol 2,3-Dioxygenase - genetics; Catechol 2,3-Dioxygenase - metabolism; Communities; Contamination; Control equipment; Decontamination; Decontamination. Miscellaneous; Degradation; Density; DNA, Fungal - genetics; DNA, Fungal - isolation & purification; Ethers; Exact sciences and technology; Fatty Acids - analysis; fungi; Gene Dosage; heterotrophs; Historical contamination; isomers; Lentinula - chemistry; Lentinula - genetics; Lentinula - growth & development; Lentinus; Lentinus (Panus) tigrinus; Metals, Heavy - analysis; Mycelium - growth & development; Mycoremediation; Phospholipids - analysis; polluted soils; Pollution; Reproduction; Reverse Transcriptase Polymerase Chain Reaction; Soil (material); Soil - analysis; Soil and sediments pollution; Soil Microbiology; Soil Pollutants - analysis; soil pollution
• ISSN: 0304-3894; 1873-3336
• DOI: 10.1016/j.jhazmat.2010.11.128

The ability of Lentinus tigrinus to grow and to degrade persistent aromatic hydrocarbons in aged contaminated soil was assessed in this study. L. tigrinus extensively colonized the soil; its degradation activity after 60d incubation at 28°C, however, was mostly limited to dichloroaniline isomers, polychlorinated benzenes and diphenyl ether while the fungus was unable to deplete 9,10-anthracenedione and 7-H-benz[DE]anthracene-7-one which were the major soil contaminants. Although clean-up levels were limited, both density of cultivable heterotrophic bacteria and richness of the resident bacterial community in L. tigrinus microcosms (LtM) increased over time to a significantly larger extent than the respective amended incubation controls (1.9×109CFUg−1vs. 1.0×109CFUg−1 and 37 vs. 16, respectively). Naphthalene- and catechol 2,3-dioxygenase gene copy numbers, however, decreased over time at a higher rate in LtM than in incubation controls likely due to a higher stimulation on heterotrophs than xenobiotics-degrading community members.