Recombinant pre-pro-Concanavalin A (jack bean) is stable but of low solubility

The cDNA for pre-pro-Concanavalin A (pre-pro-ConA) was cloned into the cytoplasmic expression vector pKK233-2 to give rise to pCONEXP2 which was used to express the lectin precursor. Pre-pro-ConA is stable and is not transposed and ligated to form the mature protein. No signal peptide removal is obs...

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Bibliographic Details
Published in:Journal of biosciences 2001-12, Vol.26 (5), p.635-640
Main Authors: Dincturk, H B, Dunn, A J, Jones, D H
Format: Article
Language:English
Subjects:
Beans
Blotting, Western
Cloning
Cloning, Molecular
Concanavalin A - chemistry
Concanavalin A - genetics
Fabaceae - chemistry
Legumes
Plant Lectins
Protein Precursors - chemistry
Protein Precursors - genetics
Proteins
Recombinant Proteins - chemistry
Recombinant Proteins - genetics
Solubility
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Summary:The cDNA for pre-pro-Concanavalin A (pre-pro-ConA) was cloned into the cytoplasmic expression vector pKK233-2 to give rise to pCONEXP2 which was used to express the lectin precursor. Pre-pro-ConA is stable and is not transposed and ligated to form the mature protein. No signal peptide removal is observed. The solubility of pre-pro-ConA could not be increased by guanidine hydrochloride denaturation/dilution treatment.
ISSN:0250-5991
0973-7138
DOI:10.1007/BF02704761