Ecdysteroid biosynthesis in workers of the European honeybee Apis mellifera L

• Published in: Insect biochemistry and molecular biology 2011-05, Vol.41 (5), p.283-293
• Main Authors: Yamazaki, Yurika, Kiuchi, Makoto, Takeuchi, Hideaki, Kubo, Takeo
• Format: Article
• Language: English
• Subjects: animal ovaries; Animals; Apis mellifera; Bees - cytology; Bees - enzymology; Bees - genetics; biosynthesis; Chromatography, High Pressure Liquid; culture media; Culture Media, Conditioned; Cytochrome P-450 Enzyme System - metabolism; ecdysone; Ecdysone - biosynthesis; Ecdysone - genetics; Ecdysteroid; Ecdysterone - biosynthesis; Ecdysterone - genetics; Europe; fat body; Fat Body - cytology; Fat Body - enzymology; Female; Gene Expression Regulation, Enzymologic - physiology; Honeybee; Hormone production; Insect Proteins - genetics; Insect Proteins - metabolism; mushroom bodies; organ culture; Organ Culture Techniques; Organ Specificity; Radioimmunoassay; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - analysis; RNA, Messenger - biosynthesis; Signal Transduction - physiology; Steroid Hydroxylases - genetics; Steroid Hydroxylases - metabolism; worker honey bees
• ISSN: 0965-1748; 1879-0240
• DOI: 10.1016/j.ibmb.2011.01.005

We previously reported preferential expression of genes for ecdysteroid signaling in the mushroom bodies of honeybee workers, suggesting a role of ecdysteroid signaling in regulating honeybee behaviors. The organs that produce ecdysteroids in worker honeybees, however, remain unknown. We show here that the expression of neverland and Non- molting glossy/ shroud, which are involved in early steps of ecdysteroid synthesis, was enhanced in the ovary, while the expression of CYP306A1 and CYP302A1, which are involved in later steps of ecdysone synthesis, was enhanced in the brain, and the expression of CYP314A1, which is involved in converting ecdysone into active 20-hydroxyecdysone (20E), was enhanced in the brain, fat body, and ovary. In in vitro organ culture, a significant amount of ecdysteroids was detected in the culture medium of the brain, fat body, and hypopharyngeal glands. The ecdysteroids detected in the culture medium of the fat body were identified as ecdysone and 20E. These findings suggest that, in worker honeybees, cholesterol is converted into intermediate ecdysteroids in the ovary, whereas ecdysone is synthesized and secreted mainly by the brain and converted into 20E in the brain and fat body. [Display omitted] ► Neverland and nm- g/ sro were expressed strongly in the ovary of the worker honeybees. ► CYP306A1 and CYP302A1 were expressed strongly in the brain. ► In contrast, CYP314A1 was expressed strongly in the brain, fat body and ovary. ► Ecdysteroids were detected in the culture medium of brain, fat body and hypopharyngeal glands. ► This is the first report of organs with a potential to produce ecdysteroid in adult worker honeybees.