Radiation-induced Reduction of Osteoblast Differentiation in C2C12 cells

Therapeutic radiation causes bone damage and may increase fracture risks in treatment for head-and-neck cancer and in pelvic irradiation. These properties can also be used for prevention of heterotopic ossification in hip arthroplasty. To evaluate the effects of ionizing radiation on osteoblast diff...

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Bibliographic Details
Published in:JOURNAL OF RADIATION RESEARCH 2007, Vol.48 (6), p.515-521
Main Authors: Sakurai, Tomonori, Sawada, Yohei, Yoshimoto, Miwa, Kawai, Miyuki, Miyakoshi, Junji
Format: Article
Language:English
Subjects:
Animals
Anticoagulants
Cell Differentiation - radiation effects
Cell Line
Collagen
Dose-Response Relationship, Radiation
Health aspects
Ionizing radiation
Mice
Myoblasts - cytology
Myoblasts - physiology
Myoblasts - radiation effects
Osteoblasts - cytology
Osteoblasts - physiology
Osteoblasts - radiation effects
Osteogenesis - physiology
Osteogenesis - radiation effects
Phosphatases
Radiation Dosage
RNA
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Summary:Therapeutic radiation causes bone damage and may increase fracture risks in treatment for head-and-neck cancer and in pelvic irradiation. These properties can also be used for prevention of heterotopic ossification in hip arthroplasty. To evaluate the effects of ionizing radiation on osteoblast differentiation, C2C12 cells were directed into an osteogenic lineage by treatment with a combination of bone morphogenic protein 2 (BMP-2) (100 ng/ml) and heparin (30μg/ml) 6 h after irradiation (2 and 4 Gy). Osteoblast differentiation was evaluated based on alkali phosphatase (ALP) activity and expression of mRNA encoding ALP and collagen type I. Ionizing radiation suppressed the growth of C2C12 cells and decreased expression of ALP and collagen type I mRNAs with concomitant reduction of the ALP activity. Although further studies are needed to elucidate the molecular mechanism, our findings suggest that ionizing radiation at therapeutic doses interferes with bone formation by reducing ALP activity and expression of mRNA encoding ALP and collagen type I.
ISSN:0449-3060
1349-9157
1349-9157
DOI:10.1269/jrr.07012