An amphiphilic fluorescent probe for the visualization of histamine in living cells

A novel fluorescent probe composed of two moieties, Nile Red and an iminodiacetic acid–Ni2+ complex, for the detection of histamine in living cells is described. The probe was successfully applied to visualizing histamine in RAW264 cells, representing the first demonstration of the imaging of histam...

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Bibliographic Details
Published in:Bioorganic & medicinal chemistry letters 2010-11, Vol.20 (22), p.6708-6711
Main Authors: Seto, Daisuke, Soh, Nobuaki, Nakano, Koji, Imato, Toshihiko
Format: Article
Language:English
Subjects:
Analytical biochemistry: general aspects, technics, instrumentation
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Cell Line
Fluorescence imaging
Fluorescent Dyes - metabolism
Fundamental and applied biological sciences. Psychology
Histamine
Histamine - metabolism
Ligand exchange
Macrophages - metabolism
Mice
Microscopy, Fluorescence
Nile Red
Sensor molecule
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Summary:A novel fluorescent probe composed of two moieties, Nile Red and an iminodiacetic acid–Ni2+ complex, for the detection of histamine in living cells is described. The probe was successfully applied to visualizing histamine in RAW264 cells, representing the first demonstration of the imaging of histamine itself in living cells.
ISSN:0960-894X
1464-3405
DOI:10.1016/j.bmcl.2010.09.003