Serodiagnosis of experimental sparganum infections of mice and human sparganosis by ELISA using ES antigens of Spirometra mansoni spargana

We conducted a study of serodiagnosis of experimental sparganum infections of mice and human sparganosis by enzyme-linked immunosorbent assay (ELISA) using excretory–secretory (ES) antigens of Spirometra mansoni spargana and compared the sensitivity and specificity of crude and ES antigens for detec...

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Bibliographic Details
Published in:Parasitology research (1987) 2011-06, Vol.108 (6), p.1551-1556
Main Authors: Cui, Jing, Li, Nan, Wang, Zhong Quan, Jiang, Peng, Lin, Xi Meng
Format: Article
Language:English
Subjects:
Analysis
Animals
Antibacterial agents
Antibodies, Helminth - blood
Antibodies, Helminth - immunology
Antigens
Antigens, Helminth - immunology
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Cestode diseases
Echinococcus
Enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Frogs
Fundamental and applied biological sciences. Psychology
General aspects
General aspects and techniques. Study of several systematic groups. Models
Humans
Immunoglobulin G
Immunoglobulin G - blood
Immunoglobulin G - immunology
Immunology
Infection
Invertebrates
Medical Microbiology
Medical research
Medicine, Experimental
Mice
Microbiology
Monoclonal antibodies
Original Paper
Schistosoma
Schistosoma mansoni
Serologic Tests
Sparganosis - blood
Sparganosis - diagnosis
Sparganosis - immunology
Sparganum - immunology
Spirometra
Toxoplasma gondii
Trichinella spiralis
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Summary:We conducted a study of serodiagnosis of experimental sparganum infections of mice and human sparganosis by enzyme-linked immunosorbent assay (ELISA) using excretory–secretory (ES) antigens of Spirometra mansoni spargana and compared the sensitivity and specificity of crude and ES antigens for detecting the specific anti-sparganum IgG antibodies. By crude antigen ELISA and ES antigen ELISA, anti-sparganum IgG was detected in all of 30 serum samples of the infected mice; no cross-reactions were observed in serum samples of the mice infected with Trichinella spiralis , Schistosoma japanicum , Toxoplasma gondii , and normal mice. Anti-sparganum IgG was detected by ES antigen ELISA in sera of mice infected with one, two, four, six, and eight spargana at 3 weeks post-infection (wpi), with a detection rate of 100%, and lasted to 18 wpi when the experiment was ended. The difference in anti-sparganum antibody levels among five groups of the infected mice was statistically significant ( F  = 245.296, p  
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-010-2206-2