Influence of organic zinc supplementation on the antioxidant status and immune responses of broilers challenged with Eimeria tenella

• Published in: Poultry science 2011-06, Vol.90 (6), p.1220-1226
• Main Authors: Bun, S.D, Guo, Y.M, Guo, F.C, Ji, F.J, Cao, H
• Format: Article
• Language: English
• Subjects: Age; Animal Feed - analysis; Animal Nutritional Physiological Phenomena; Animals; Antioxidants; Antioxidants - metabolism; birds; Chelates; Chickens; chicks; Coccidiosis - immunology; Coccidiosis - parasitology; Coccidiosis - veterinary; Concanavalin A; diet; Diet - veterinary; Dietary Supplements; Diets; Eimeria tenella; Eimeria tenella - physiology; Glutathione peroxidase; growth performance; Immune response; Immunoglobulin A; Lipid peroxidation; Lipopolysaccharides; lymphocyte proliferation; Lymphocytes; Lymphocytes - cytology; Lymphocytes - physiology; Male; Methionine; Mitogens; Oocysts; Oxidative Stress; Poultry; Poultry Diseases - drug therapy; Poultry Diseases - immunology; Poultry Diseases - parasitology; Superoxide dismutase; Zinc; Zinc - therapeutic use
• ISSN: 0032-5791; 1525-3171
• DOI: 10.3382/ps.2010-01308

An experiment was conducted to investigate the influence of Zn supplementation on the performance, antioxidant status, and immune responses of broilers challenged with Eimeria tenella. A total of 384 male broilers (1 d old) were assigned to 8 treatments consisting of 8 replicates of 6 chicks each. A basal corn-soybean meal diet (29.6 mg of Zn/kg) was supplemented with methionine hydroxyl analog-Zn chelate at 0, 20, 40, and 60 mg/kg of diet. At 21 d of age, birds were orally gavaged with 1.5 x 10⁴ sporulated E. tenella oocysts. Dietary Zn supplementation had no effect on growth performance of either the challenged or nonchallenged birds. Activities of Cu-Zn superoxide dismutase and glutathione peroxidase were increased (P < 0.001) with increasing Zn levels in both the challenged and nonchallenged groups. Lipid peroxidation tended to be reduced (P = 0.08) at Zn inclusion of 20 and 40 mg/kg. In vitro lymphocyte proliferation responses to mitogen concanavalin A and LPS were not influenced by dietary Zn or challenge. The main effects of Zn level and challenge were significant for secretory IgA on d 28 (P < 0.01) and 35 (P < 0.001). During both periods, secretory IgA of birds receiving dietary Zn supplementation was higher (P < 0.05) than that of those receiving no Zn supplementation. Birds fed Zn supplementation excreted fewer oocysts in the excreta than those receiving no Zn supplement (P < 0.001). Results indicated that organic Zn supplementation reduced oxidative stress and improved some immune responses irrespective of whether birds were healthy or challenged with E. tenella.