Facile Synthesis of Monodispersed Mesoporous Silica Nanoparticles with Ultralarge Pores and Their Application in Gene Delivery

Among various nanoparticles, the silica nanoparticle (SiNP) is an attractive candidate as a gene delivery carrier due to advantages such as availability in porous forms for encapsulation of drugs and genes, large surface area to load biomacromolecules, biocompatibility, storage stability, and easy p...

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Bibliographic Details
Published in:ACS nano 2011-05, Vol.5 (5), p.3568-3576
Main Authors: Kim, Mi-Hee, Na, Hee-Kyung, Kim, Young-Kwan, Ryoo, Soo-Ryoon, Cho, Hae Sung, Lee, Kyung Eun, Jeon, Hyesung, Ryoo, Ryong, Min, Dal-Hee
Format: Article
Language:English
Subjects:
Diffusion
HeLa Cells
Humans
Materials Testing
Nanocapsules - chemistry
Plasmids - administration & dosage
Plasmids - chemistry
Plasmids - genetics
Porosity
Silicon Dioxide - chemistry
Transfection - methods
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Summary:Among various nanoparticles, the silica nanoparticle (SiNP) is an attractive candidate as a gene delivery carrier due to advantages such as availability in porous forms for encapsulation of drugs and genes, large surface area to load biomacromolecules, biocompatibility, storage stability, and easy preparation in large quantity with low cost. Here, we report on a facile synthesis of monodispersed mesoporous silica nanoparticles (MMSN) possessing very large pores (>15 nm) and application of the nanoparticles to plasmid DNA delivery to human cells. The aminated MMSN with large pores provided a higher loading capacity for plasmids than those with small pores (∼2 nm), and the complex of MMSN with plasmid DNA readily entered into cells without supplementary polymers such as cationic dendrimers. Furthermore, MMSN with large pores could efficiently protect plasmids from nuclease-mediated degradation and showed much higher transfection efficiency of the plasmids encoding luciferase and green fluorescent protein (pLuc, pGFP) compared to MMSN with small pores (∼2 nm).
ISSN:1936-0851
1936-086X
DOI:10.1021/nn103130q