Yeasts from Macrobrachium amazonicum: a focus on antifungal susceptibility and virulence factors of Candida spp

• Published in: FEMS microbiology ecology 2011-05, Vol.76 (2), p.268-277
• Main Authors: Brilhante, Raimunda S.N., Paiva, Manoel A.N., Sampaio, Célia M.S., Teixeira, Carlos E.C., Castelo-Branco, Débora S.C.M., Leite, João J.G., Moreira, Camila A., Silva, Liliane P., Cordeiro, Rossana A., Monteiro, André J., Sidrim, José J.C., Rocha, Marcos F.G.
• Format: Article
• Language: English
• Subjects: Animal, plant and microbial ecology; Animals; Antifungal Agents - pharmacology; antifungal susceptibility; Aquaculture; Aspergillus; Aspergillus - classification; Aspergillus - drug effects; Aspergillus - isolation & purification; Aspergillus - pathogenicity; Biological and medical sciences; Candida; Candida - classification; Candida - drug effects; Candida - isolation & purification; Candida - pathogenicity; Candida famata; Cladosporium; Cladosporium - classification; Cladosporium - drug effects; Cladosporium - isolation & purification; Cladosporium - pathogenicity; Comparative studies; Cultivation; Ecology; Freshwater; Fundamental and applied biological sciences. Psychology; Macrobrachium amazonicum; Medical research; Microbial ecology; Microbial Sensitivity Tests; Microbiology; Miscellaneous; Mycology; Older people; Palaemonidae - microbiology; Penicillium; Penicillium - classification; Penicillium - drug effects; Penicillium - isolation & purification; Penicillium - pathogenicity; virulence factors; Virulence Factors - metabolism; Water Microbiology; Yeast diversity; Yeasts
• ISSN: 0168-6496; 1574-6941
• DOI: 10.1111/j.1574-6941.2011.01050.x

Abstract In the present study, it was sought to compare yeast microbiota of wild and captive Macrobrachium amazonicum and evaluate the antifungal susceptibility and production of virulence factors by the recovered isolates of Candida spp. Additionally, cultivation water was monitored for the presence of fungi. Overall, 26 yeast isolates belonging to three genera and seven species were obtained, out of which 24 were Candida spp., with Candida famata as the most prevalent species for both wild and captive prawns. From cultivation water, 28 isolates of filamentous fungi were obtained, with Penicillium spp., Cladosporium spp. and Aspergillus spp. as the most frequent genera. Eight out of 24 Candida spp. isolates were resistant to azole derivatives, out of which four were recovered from wild-harvested prawns. As for production of virulence factors, three (12.5%) and eight (33.3%) isolates presented phospholipase and protease activity, respectively. This is the first comparative study between wild and captive prawns and the first report on yeast microbiota of M. amazonicum. The most relevant finding was the high percentage of resistant Candida spp., including from wild individuals, which suggests the occurrence of an environmental imbalance in the area where these prawns were captured.