Overpressured Layer Chromatography (OPLC) – A Flexible Tool of Analysis and Isolation

This review briefly summarizes the overpressured layer chromatography (OPLC) technique and its progress from the beginning until today. Some theoretical aspects, important technical and methodological solutions, as well as analytical separations and isolations are demonstrated covering the last 30 y...

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Bibliographic Details
Published in:Natural product communications 2011-05, Vol.6 (5), p.719-732
Main Authors: Mincsovics, Emil, Tyihák, Ernő
Format: Article
Language:English
Subjects:
Biological Assay
Biological Products - isolation & purification
Chromatography, Thin Layer - instrumentation
Chromatography, Thin Layer - methods
Chromatography, Thin Layer - trends
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Summary:This review briefly summarizes the overpressured layer chromatography (OPLC) technique and its progress from the beginning until today. Some theoretical aspects, important technical and methodological solutions, as well as analytical separations and isolations are demonstrated covering the last 30 years. The infusion and transfusion OPLC operations, as well as their combination, and their off-line and online processes, and off-line and online hyphenations for detection and structure elucidation are presented. The combination of OPLC separation with biological detection by direct bioautography and BioArena as an important solution touches the potential of analysis and isolation based on biological activity.
ISSN:1934-578X
1555-9475
DOI:10.1177/1934578X1100600528