Chloride-associated adaptive response in aerobic methylotrophic dichloromethane-utilising bacteria

Aerobic methylotrophic bacteria able to grow with dichloromethane (DCM) as the sole carbon and energy source possess a specific glutathione S‐transferase, DCM dehalogenase, which transforms DCM to formaldehyde, used for biomass and energy production, and hydrochloric acid, which is excreted. Evidenc...

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Published in:Journal of basic microbiology 2011-06, Vol.51 (3), p.296-303
Main Authors: Torgonskaya, Maria L., Doronina, Nina V., Hourcade, Edith, Trotsenko, Yuri A., Vuilleumier, Stéphane
Format: Article
Language:English
Subjects:
Aerobiosis
Chloride
Chlorides - metabolism
Dehalogenation
Dichloromethane
Lyases - metabolism
Methylene Chloride - metabolism
Methylobacterium extorquens - drug effects
Methylobacterium extorquens - metabolism
Methylobacterium extorquens - physiology
Methylocystaceae - drug effects
Methylocystaceae - metabolism
Methylocystaceae - physiology
Methylotrophy
Microscopy, Electron
Photoelectron Spectroscopy
Salinity
Stress, Physiological
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Summary:Aerobic methylotrophic bacteria able to grow with dichloromethane (DCM) as the sole carbon and energy source possess a specific glutathione S‐transferase, DCM dehalogenase, which transforms DCM to formaldehyde, used for biomass and energy production, and hydrochloric acid, which is excreted. Evidence is presented for chloride‐specific responses for three DCM‐degrading bacteria, Methylobacterium extorquens DM4, Methylopila helvetica DM6 and Albibacter methylovorans DM10. Chloride release into the medium was inhibited by sodium azide and m ‐chlorophenylhydrazone, suggesting an energy‐dependent process. In contrast, only nigericin affected chloride excretion in Mb. extorquens DM4 and Mp. helvetica DM6, while valinomycin had the same effect in A. methylovorans DM10 only. Chloride ions stimulated DCM‐dependent induction of DCM dehalogenase expression for Mp. helvetica DM6 and A. methylovorans DM10, and shortened the time for onset of chloride release into the medium. Striking chloride‐containing structures were observed by electron microscopy and X‐ray microanalysis on the cell surface of Mp. helvetica DM6 and A. methylovorans DM10 during growth with DCM, and with methanol in medium supplemented with sodium chloride. Taken together, these data suggest the existence of both general and specific chloride‐associated adaptations in aerobic DCM‐degrading bacteria. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
ISSN:0233-111X
1521-4028
DOI:10.1002/jobm.201000280