A role for the anaphase promoting complex in hormone regulation

To increase our knowledge of anaphase promoting complex (APC/C) function during plant development, we characterized an Arabidopsis thaliana T-DNA-insertion line where the T-DNA fell within the 5′ regulatory region of the APC10 gene. The insert disrupted endogenous expression, resulting in overexpres...

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Bibliographic Details
Published in:Planta 2011-06, Vol.233 (6), p.1223-1235
Main Authors: Lindsay, Donna L., Bonham-Smith, Peta C., Postnikoff, Spike, Gray, Gordon R., Harkness, Troy A. A.
Format: Article
Language:English
Subjects:
Agriculture
Anaphase-Promoting Complex-Cyclosome
Arabidopsis - genetics
Arabidopsis - metabolism
Auxins
Base Sequence
Biological and medical sciences
Biomedical and Life Sciences
Cell cycle
Cotyledon - cytology
Cotyledons
Deoxyribonucleic acid
DNA
DNA, Bacterial - genetics
Ecology
Ethylenes - biosynthesis
Forestry
Fundamental and applied biological sciences. Psychology
Gene expression regulation
Gene Expression Regulation, Plant
Genes, Plant
Hormones
Hypocotyls
Indoleacetic Acids - metabolism
Life Sciences
Original Article
Phenotypes
Plant development
Plant Growth Regulators - genetics
Plant Growth Regulators - metabolism
Plant Sciences
Plants
RNA, Messenger - genetics
RNA, Messenger - metabolism
Seedlings
Seedlings - cytology
Ubiquitin-Protein Ligase Complexes - metabolism
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Summary:To increase our knowledge of anaphase promoting complex (APC/C) function during plant development, we characterized an Arabidopsis thaliana T-DNA-insertion line where the T-DNA fell within the 5′ regulatory region of the APC10 gene. The insert disrupted endogenous expression, resulting in overexpression of APC10 mRNA from the T-DNA- internal CaMV 35S promoter, and increased APC10 protein. Overexpression of APC10 produced phenotypes resembling those of known auxin and ethylene mutants, and increased expression of two tested auxin-regulated genes, small auxin up RNA (SAUR) 15 and SAUR24. Taken together, our data suggests that elevated APC10 likely mimics auxin and ethylene sensitive phenotypes, expanding our understanding of proteolytic processes in hormone regulation of plant development.
ISSN:0032-0935
1432-2048
DOI:10.1007/s00425-011-1374-3