A Good Breath of Oxygen for Beta-Like Cells Obtained From Porcine Exocrine Pancreatic Tissue

• Published in: Transplantation proceedings 2011-05, Vol.43 (4), p.1173-1177
• Main Authors: Gioviale, M.C, Damiano, G, Cacciabaudo, F, Palumbo, V.D, Bellavia, M, Cassata, G, Spinelli, G, Buscemi, G, Lo Monte, A.I
• Format: Article
• Language: English
• Subjects: Adenosine - pharmacology; Allopurinol - pharmacology; Animals; Biological and medical sciences; Cell Differentiation; Cell Separation; Cell Survival - drug effects; Cells, Cultured; Cryopreservation; Culture Media - metabolism; Female; Fluorocarbons - pharmacology; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Glucose - metabolism; Glutathione - pharmacology; Insulin - metabolism; Insulin - pharmacology; Insulin-Secreting Cells - drug effects; Insulin-Secreting Cells - metabolism; Laparoscopy; Medical sciences; Organ Preservation Solutions - pharmacology; Oxygen - metabolism; Pancreas, Exocrine - cytology; Pancreas, Exocrine - drug effects; Pancreas, Exocrine - metabolism; Pancreatectomy; Raffinose - pharmacology; Surgery; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Swine; Time Factors; Tissue and Organ Harvesting - methods; Tissue, organ and graft immunology
• ISSN: 0041-1345; 1873-2623
• DOI: 10.1016/j.transproceed.2011.02.039

Abstract Ischemia is the most important factor that affects organ survival during harvesting. The two-layer method (TLM) is one of several cold storage solutions that seeks to preserve organs and cells avoiding in vivo and in vitro ischemia. We compared the retrieval of beta-like elements from exocrine pancreatic cells using TLM versus University of Wisconsin (UW) solutions. For this purpose pancreata laparoscopically harvested from 20 female pigs were preserved in UW solution or TLM before digestion. The resulting exocrine cells were divided into 2 groups: the first was cultured in a designed medium to allow differentiation into beta-like cells and the second was cryopreserved before the differentiation process at −196°C for 8 weeks before culture in the same medium. The results revealed that TLM was better than UW as a preservation solution in terms of beta-cell viability and insulin secretion. We suggest that the use of TLM solution allows one to obtain less damaged cells for research purposes.