Linamarin Sensors:  Interference-Based Sensing of Linamarin Using Linamarase and Peroxidase

An interference-based linamarin sensor is developed. Horseradish peroxidase (HRP) is adsorbed on a pyrolytic graphite (PG) electrode, and then linamarase from cassava is cross-linked with glutaraldehyde on the electrode surface. The prepared bienzyme electrode is poised at −300 mV vs Ag/AgCl for 40...

Full description

Saved in:
Bibliographic Details
Published in:Analytical chemistry (Washington) 1996-09, Vol.68 (17), p.2946-2950
Main Authors: Tatsuma, Tetsu, Tani, Koichiro, Oyama, Noboru, Yeoh, Hock-Hin
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biosensors
Biotechnology
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Various methods and equipments
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An interference-based linamarin sensor is developed. Horseradish peroxidase (HRP) is adsorbed on a pyrolytic graphite (PG) electrode, and then linamarase from cassava is cross-linked with glutaraldehyde on the electrode surface. The prepared bienzyme electrode is poised at −300 mV vs Ag/AgCl for 40 s to reduce dissolved O2 to H2O2 at the PG surface. The potential is then stepped to 0 mV, at which point the accumulated H2O2 is reduced, though the O2 reduction does not proceed. Since the H2O2 reduction is catalyzed by HRP, the transient cathodic current is inhibited by cyanide, which is liberated from linamarin by linamarase. Therefore, the transient current is a function of the linamarin concentration. This sensor responds to 1 × 10-5−5 × 10-3 M linamarin and can estimate a linamarin concentration of a cassava extract.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac9601324