Role of T cell competition in the induction of cytotoxic T lymphocyte activity during viral vector-based immunization regimens

Abstract T cell competition between antigen- and vector-specific T cells may determine the outcome of viral vector-based immunization regimens, as we previously proposed. Here, we unravelled the interplay between antigen- and vector-specific immunity, using recombinant Semliki Forest virus (rSFV). P...

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Bibliographic Details
Published in:Vaccine 2010-06, Vol.28 (26), p.4275-4282
Main Authors: Lambeck, Annechien J.A, Nijman, Hans W, Hoogeboom, Baukje Nynke, Regts, Joke, de Mare, Arjan, Wilschut, Jan, Daemen, Toos
Format: Article
Language:English
Subjects:
Allergy and Immunology
Animals
Antibody Specificity
Applied microbiology
Biological and medical sciences
Cancer
Cell Line
Cloning
Competition
Cricetinae
Epitopes - immunology
Female
Fundamental and applied biological sciences. Psychology
Human papillomavirus
Immunity, Cellular
Immunization
Immunization, Secondary
Immunogenicity
Influenza virus
Lymphocyte Activation
Lymphocytes
Medical research
Mice
Mice, Inbred C57BL
Microbiology
Miscellaneous
Neural networks
Orthomyxoviridae - immunology
Papillomaviridae - immunology
Peptides
Plasmids
Proteins
RNA Replicase - immunology
Semliki Forest virus
Semliki forest virus - immunology
Studies
T cell competition
T cell receptors
T-Lymphocytes, Cytotoxic - immunology
Transgenes - immunology
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Viral vector
Virology
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Summary:Abstract T cell competition between antigen- and vector-specific T cells may determine the outcome of viral vector-based immunization regimens, as we previously proposed. Here, we unravelled the interplay between antigen- and vector-specific immunity, using recombinant Semliki Forest virus (rSFV). Priming of mice with rSFV, encoding LacZ (SFVLacZ), or with empty rSFV strongly suppressed subsequent induction of ovalbumin or Human Papilloma virus (HPV) E6/E7-specific CTL activity by a booster with SFVeOVA or SFVeE6,7, respectively. Yet, priming with irradiated, i.e. replication-defective, SFVLacZ did not affect subsequent CTL induction, indicating that the interfering vector-specific immunity was directed against the viral replicase. However, immune responses against the strongly immunogenic nucleoprotein of influenza virus encoded by SFV were less severely affected by priming with SFVLacZ. Thus the outcome of heterologous prime-boost immunizations appears to depend on the immunogenicity of the respective antigens.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2010.04.033