Estrogen DownaRegulates mRNA Encoding the Exocytotic Protein SNAPa25 in the Rat Pituitary Gland

Exocytosis is dependent on specific proteins that are located at the secretory granule membrane, in the cytoplasm or at the plasma membrane. The mRNA expression of synaptosomal-associated protein of 25kDa (SNAP-25) isoforms SNAP-25a and SNAP-25b, vesicle associated membrane protein (VAMP) 2, mammali...

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Bibliographic Details
Published in:Journal of neuroendocrinology 1998-03, Vol.10 (3), p.157-163
Main Authors: Jacobsson, Gunilla, Razani, Haleh, Ogren, SvenaOve, Meister, BjAPGrn
Format: Article
Language:English
Subjects:
Cytoplasm
Estradiol
Estrogens
Exocytosis
Gene expression
Leaves
Membrane proteins
Membrane vesicles
Menopause
Ovariectomy
Pituitary
Plasma membranes
Secretory vesicles
SNAP-25 protein
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Summary:Exocytosis is dependent on specific proteins that are located at the secretory granule membrane, in the cytoplasm or at the plasma membrane. The mRNA expression of synaptosomal-associated protein of 25kDa (SNAP-25) isoforms SNAP-25a and SNAP-25b, vesicle associated membrane protein (VAMP) 2, mammalian homologue of unc-18 (munc-18) and Hrs-2 was studied in the pituitary of ovariectomized rats after subcutaneous insertion of capsules containing estrogen or placebo using in situ hybridization. Estrogen treatment (0.25mg estradiol) significantly decreased SNAP-25a (32%; 10%) and SNAP-25b (25%; 22%) mRNA levels in the anterior and intermediate lobes, respectively, whereas VAMP-2, munc-18 and Hrs-2 mRNA levels remained unchanged. The results suggest that estrogen selectively regulates SNAP-25 transcription in the pituitary gland, but leaves VAMP-2, munc-18 and Hrs-2 mRNA levels unaffected.
ISSN:0953-8194
1365-2826
DOI:10.1046/j.1365-2826.1998.00178.x