Directed evolution of angiotensin II-inhibiting peptides using a microbead display

Angiotensin II (ang II), an octapeptide (DRVYVHPF), can regulate blood pressure by binding specifically to its receptor, AT1. A peptide (VVIVIY) in the first transmembrane of AT1 has been found, via peptide array technology, to have an affinity for ang II. In this study, the peptide P2, which contai...

Full description

Saved in:
Bibliographic Details
Published in:Journal of bioscience and bioengineering 2010-04, Vol.109 (4), p.411-417
Main Authors: Gan, Rui, Furuzawa, Seiji, Kojima, Takaaki, Kanie, Kei, Kato, Ryuji, Okochi, Mina, Honda, Hiroyuki, Nakano, Hideo
Format: Article
Language:English
Subjects:
Amino Acid Sequence
AMINO ACID SEQUENCES
ANGIOTENSIN
Angiotensin II
Angiotensin II - antagonists & inhibitors
Angiotensin II - genetics
Angiotensin II - metabolism
ANGIOTENSINA
ANGIOTENSINE
Animals
Base Sequence
Biological and medical sciences
Biotechnology
Cell-free protein synthesis
Directed evolution
Directed Molecular Evolution - methods
DNA Probes - genetics
EVOLUCION
EVOLUTION
Fundamental and applied biological sciences. Psychology
http://www.fao.org/aos/agrovoc#c_2745
http://www.fao.org/aos/agrovoc#c_34079
http://www.fao.org/aos/agrovoc#c_36006
http://www.fao.org/aos/agrovoc#c_37202
http://www.fao.org/aos/agrovoc#c_5014
http://www.fao.org/aos/agrovoc#c_5691
http://www.fao.org/aos/agrovoc#c_6258
In Vitro Techniques
Kinetics
Methods. Procedures. Technologies
Microbead display
Microspheres
MUTACION
Mutagenesis, Site-Directed
MUTATION
Oligopeptides - genetics
Oligopeptides - metabolism
Oligopeptides - pharmacology
PCR
PEPTIDE
Peptide Library
PEPTIDES
PEPTIDOS
Protein engineering
PROTEIN SYNTHESIS
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Recombinant Proteins - pharmacology
SECUENCIAS DE AMINOACIDOS
SEQUENCE D'ACIDES AMINES
Single-molecule PCR
SINTESIS DE PROTEINAS
SYNTHESE PROTEIQUE
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Angiotensin II (ang II), an octapeptide (DRVYVHPF), can regulate blood pressure by binding specifically to its receptor, AT1. A peptide (VVIVIY) in the first transmembrane of AT1 has been found, via peptide array technology, to have an affinity for ang II. In this study, the peptide P2, which contained the VVIVIY sequence, was mutated and screened using microbead display technology that utilized emulsion PCR and cell-free protein synthesis. After one round of screening, the binding activities of collected mutants were estimated using flow cytometry and a peptide array. Two of these exhibited improved association rate constants to ang II, compared to the P2 peptide.
ISSN:1389-1723
1347-4421
DOI:10.1016/j.jbiosc.2009.10.009