Psychological stress during exercise: Lymphocyte subset redistribution in firefighters

• Published in: Physiology & behavior 2010-10, Vol.101 (3), p.320-326
• Main Authors: Huang, Chun-Jung, Webb, Heather E, Garten, Ryan S, Kamimori, Gary H, Acevedo, Edmund O
• Format: Article
• Language: English
• Subjects: Adult; Analysis of Variance; Behavioral psychophysiology; Bicycle ergometry; Biological and medical sciences; Catecholamines; Computer Simulation; Decision Making - physiology; Epinephrine - blood; Exercise; Exercise Test; Firefighters; Fires; Fundamental and applied biological sciences. Psychology; Heart rate; Heart Rate - physiology; Humans; Lymphocyte Subsets - classification; Lymphocyte Subsets - immunology; Lymphocyte Subsets - physiology; Lymphocytes; Male; Norepinephrine - blood; Physical Exertion - physiology; Psychiatry; Psychological stress; Psychology. Psychoanalysis. Psychiatry; Psychology. Psychophysiology; Reference Values; Statistics, Nonparametric; Stress hormone; Stress, Physiological - immunology; Stress, Physiological - physiology; Stress, Psychological - immunology; Stress, Psychological - metabolism; Sympathetic Nervous System - immunology; Sympathetic Nervous System - physiology
• ISSN: 0031-9384; 1873-507X
• DOI: 10.1016/j.physbeh.2010.05.018

Abstract The purpose of this study examined the changes in heart rate (HR), catecholamines (NE, EPI) and percentages of blood lymphocyte subsets (CD3+ T cells, CD3+ CD4+ helper T cells, CD3+ CD8+ cytotoxic T cells, CD3− CD56+ NK cells, CD4/CD8 ratio, CD19+ B cells, and total lymphocytes [NK cells+ T cells+ B cells]) in firefighters exposed to a computerized firefighting strategies and tactics decision-making challenge while participating in moderate intensity exercise. Furthermore, this study also examined the possible relationships between catecholamines (NE and EPI) and blood lymphocyte subsets following combined mental and physical challenge. Ten professional male firefighters participated in two counterbalanced exercise conditions on a cycle ergometer: (1) 37 min of cycle ergometry at 60% VO2max (exercise alone condition; EAC) and (2) 37 min of cycle ergometry at 60% VO2max along with 20 min of a computerized firefighting strategies and tactics decision-making challenge (firefighting strategies condition; FSC). FSC elicited significantly greater HR, NE, and EPI when compared to EAC. Both EAC and FSC elicited increases in CD3− CD56+ NK cells. The percentages of CD3+ T cells, CD3+ CD4+ helper T cells, CD4/CD8 ratio, CD19+ B cells, and total lymphocytes were lower immediately following both conditions. Following dual challenge NE AUC was negatively correlated with percentage of CD19+ B cells immediately post challenge, and HR was negatively associated with the percent change in the CD4/CD8 ratio from pre to post challenge. These elevations in NE and heart rate simultaneously in response to the dual challenge suggest greater sympathetic activation that in turn would possibly explain the alteration in the distribution of lymphocyte subsets.