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Method of clonal micropropagation of different willow species and hybrids
Conditions of cultivation and micropropagation of selected biotypes of five willow species ( Salyx dasyclados Wimm., S. caspica Pall., S. triandra L., S. purpurea L., and S. viminalis L.) and two hybrids (× S. acuminata S. and × S. palustris Host.) were optimized. Data on in vitro propagation of S....
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Published in: | Applied biochemistry and microbiology 2010-12, Vol.46 (8), p.769-775 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Conditions of cultivation and micropropagation of selected biotypes of five willow species (
Salyx dasyclados
Wimm.,
S. caspica
Pall.,
S. triandra
L.,
S. purpurea
L., and
S. viminalis
L.) and two hybrids (×
S. acuminata
S. and ×
S. palustris
Host.) were optimized. Data on in vitro propagation of
S. caspica
,
S. triandra
,
S. purpurea
together with hybrids
S. acuminata
and
S. palustris
were obtained for the first time. It has been demonstrated that the outcome of cultivation and propagation of willows strongly depends on genotypic peculiarities of initial plants. The optimal terms of isolation and sterilization of single-node segments for obtaining 50–75% of aseptic viable developing cultures were estimated. The nutritive media were selected providing induction of stem development (to 67%), their rooting (to 91%), elongation (to 3–6 cm), and multiplication (propagation coefficient of 4). The designed method (adopted to different genotypes) can be applied for obtaining aseptic in vitro cultures serving as initial plant material for genetic transformation and mass propagation of plants with new agriculturally valuable characteristics which are of interest for construction of bioenergetic plantations and for needs of the paper industry. |
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ISSN: | 0003-6838 1608-3024 |
DOI: | 10.1134/S0003683810080065 |