Influence of the electron acceptor on nitrite reductase gene ( nir) diversity in an activated sludge community

• Published in: Journal of bioscience and bioengineering 2009-11, Vol.108 (5), p.394-399
• Main Authors: Nittami, Tadashi, Magura, Takayuki, Imai, Yuko, Matsumoto, Kanji
• Format: Article
• Language: English
• Subjects: ACTIVATED SLUDGE; AGUAS RESIDUALES; Ammonia - metabolism; BACTERIA; Bacteria, Aerobic - classification; Bacteria, Aerobic - genetics; Bacteria, Aerobic - metabolism; Biological and medical sciences; Biological treatment of waters; Bioreactors; Biotechnology; BOUE ACTIVEE; Denitrification; EAU USEE; Electron acceptor; Electron Transport; Environment and pollution; Fundamental and applied biological sciences. Psychology; GENE; GENES; Genetic Variation; http://www.fao.org/aos/agrovoc#c_29521; http://www.fao.org/aos/agrovoc#c_3214; http://www.fao.org/aos/agrovoc#c_765; http://www.fao.org/aos/agrovoc#c_8308; http://www.fao.org/aos/agrovoc#c_8611; Industrial applications and implications. Economical aspects; LODOS ACTIVADOS; Methods. Procedures. Technologies; NITRITE REDUCTASE; Nitrite reductase genes ( nirK, nirS); Nitrite Reductases - chemistry; Nitrite Reductases - genetics; Nitrite Reductases - metabolism; NITRITO REDUCTASA; Sequencing batch reactor (SBR); Sewage - microbiology; Species Specificity; Various methods and equipments; WASTEWATER; Wastewater treatment
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1016/j.jbiosc.2009.05.007

Analyses of the nitrite reductase gene diversities ( nirK and nirS) in an activated sludge community fed with both nitrite and glucose were conducted. Results suggest that the topology of nirK and nirS gene fragment-based phylogenetic trees is influenced more by the available electron acceptor than by the carbon source. A denitrification reactor was operated for 53 days and a clone library constructed when the denitrifying communities in the SBR were supplied with both nitrite and glucose. Half of the nirK and nearly all the nirS gene fragments formed a cluster that was separate from a cluster containing nirK and nirS sequences derived from other communities in nitrate-fed reactors. On the other hand, nirK and nirS fragments obtained with glucose as the carbon source were similar to those detected in communities fed with other carbon sources.