Vaccination with dendritic cells charged with apoptotic/necrotic B16 melanoma induces the formation of subcutaneous lymphoid tissue

Abstract Antigen presentation by dendritic cells (DC) is of key importance for the initiation of the primary immune response. Mice vaccinated with DC charged with apoptotic/necrotic B16 cells (DC-Apo/Nec) are protected against B16 challenge. The aim of this study was to assess vaccine cell migration...

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Bibliographic Details
Published in:Vaccine 2010-11, Vol.28 (51), p.8162-8168
Main Authors: Keon, Soledad Mac, Gazzaniga, Silvina, Mallerman, Julieta, Bravo, Alicia I, Mordoh, José, Wainstok, Rosa
Format: Article
Language:English
Subjects:
Allergy and Immunology
Animals
Antigens, Neoplasm - immunology
Apoptotic cell
Applied microbiology
Biological and medical sciences
Bone marrow
Cancer
Cancer Vaccines - administration & dosage
Cancer Vaccines - immunology
Colleges & universities
Dendritic cell
Dendritic Cells - immunology
Fundamental and applied biological sciences. Psychology
Histocytochemistry
Immune response
Immune system
Injections
Injections, Subcutaneous
Lymphatic system
Lymphocytes
Lymphoid tissue
Lymphoid Tissue - physiology
Male
Medical sciences
Melanoma
Melanoma, Experimental - immunology
Melanoma, Experimental - prevention & control
Mice
Mice, Inbred C57BL
Microbiology
Migration
Recruitment
Rodents
Subcutaneous Tissue - immunology
Survival Analysis
Tumors
Vaccination
Vaccination - methods
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
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Summary:Abstract Antigen presentation by dendritic cells (DC) is of key importance for the initiation of the primary immune response. Mice vaccinated with DC charged with apoptotic/necrotic B16 cells (DC-Apo/Nec) are protected against B16 challenge. The aim of this study was to assess vaccine cell migration in our system and to find out if there is an immunological response taking place at the vaccination site. The formation of a pseudocapsule, peripheral node addresin expression in small venules, and the recruitment of a wide variety of cellular populations, including macrophages, polymorphonuclear lymphocytes, and CD8+ and CD4+ T lymphocytes found in association with DC, evidenced the formation of tertiary lymphoid tissue in the vaccination site in our experimental system.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2010.09.095