Synergic stimulation of laminin and epidermal growth factor facilitates the myoblast growth through promoting migration

The dynamic behaviors of human skeletal muscle myoblasts were investigated in the culture on a laminin-coated surface in the presence of 100 ng/ml epidermal growth factor (EGF) in medium. The coexistence of laminin and EGF caused the enhancement of myoblast migration, giving an average migration rat...

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Bibliographic Details
Published in:Journal of bioscience and bioengineering 2009-08, Vol.108 (2), p.174-177
Main Authors: Chowdhury, Shiplu Roy, Muneyuki, Yuichi, Takezawa, Yasunori, Kino-oka, Masahiro, Saito, Atsuhiro, Sawa, Yoshiki, Taya, Masahito
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biotechnology
Cell Communication - drug effects
Cell Communication - physiology
Cell Movement - drug effects
Cell Movement - physiology
Cells, Cultured
Cytokinesis
Cytokinesis - drug effects
Dose-Response Relationship, Drug
Drug Synergism
EPIDERMAL GROWTH FACTOR
Epidermal Growth Factor - agonists
Epidermal Growth Factor - pharmacology
FACTEUR DE CROISSANCE EPIDERMIQUE
FACTOR DE CRECIMIENTO EPIDERMICO
FIBRAS MUSCULARES
FIBRE MUSCULAIRE
Fundamental and applied biological sciences. Psychology
GENERO HUMANO
GENRE HUMAIN
Humans
Laminin
Laminin - agonists
Laminin - pharmacology
MANKIND
Mice
MIGRACION
MIGRATION
Muscle Fibers, Skeletal - cytology
Muscle Fibers, Skeletal - physiology
MUSCLE FIBRES
Myoblast cells
Myoblasts, Skeletal - cytology
Myoblasts, Skeletal - physiology
PROTEINAS
PROTEINE
PROTEINS
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Summary:The dynamic behaviors of human skeletal muscle myoblasts were investigated in the culture on a laminin-coated surface in the presence of 100 ng/ml epidermal growth factor (EGF) in medium. The coexistence of laminin and EGF caused the enhancement of myoblast migration, giving an average migration rate of 62.0 μm/h, which was 2.7 times that on a plain surface. This encouraged migration could be a driving force to separate the dividing cells from each other, accompanied by shortened disjunction time of daughter cells to complete cytokinesis. In addition, the synergic effect of laminin and EGF led to the promotion of myoblast growth with keeping a relatively high fraction of proliferative cells during the culture for 150 h, which is considered to arise from the reduced frequency of cell–cell contacts during cytokinesis and thereby suppressing the process towards myotube formation after cell division.
ISSN:1389-1723
1347-4421
DOI:10.1016/j.jbiosc.2009.03.005