A single-monomer derived linear-like PEI- co -PEG for siRNA delivery and silencing

Abstract Polyethylenimines (PEIs) are commonly used as a vehicle to deliver and protect siRNA, but the strong interaction still remains to be modulated for efficient siRNA release and silencing. Herein, a single-monomer derived linear-like PEI- co -PEG (LPEI- co -PEG, P2 ) was synthesized to substan...

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Bibliographic Details
Published in:Biomaterials 2011-05, Vol.32 (14), p.3647-3653
Main Authors: Tsai, Lin-Ren, Chen, Min-Hua, Chien, Chih-Te, Chen, Meng-Kai, Lin, Fong-Sian, Lin, Kurt Ming-Chao, Hwu, Yeu-Kuang, Yang, Chung-Shi, Lin, Shu-Yi
Format: Article
Language:English
Subjects:
A non-toxic vehicle
A single-monomer copolymerization
Advanced Basic Science
Animals
Biomedical materials
Cell Line, Tumor
Copolymers
Dentistry
Enhanced siRNA release and mRNA silencing
Gene Silencing - physiology
Humans
Linear-like polyethylenimine- co-poly(ethylene glycol)
LPEI- co-PEG
Magnetic Resonance Spectroscopy
Male
Mice
Microscopy, Confocal
Photoluminescence
Polyetherimides
Polyethylene Glycols - chemistry
Polyethyleneimine - analogs & derivatives
Polyethyleneimine - chemistry
RNA Interference
RNA, Small Interfering - administration & dosage
RNA, Small Interfering - chemistry
Segments
Spectroscopy, Fourier Transform Infrared
Synchrotron X-rays irradiation
Synchrotrons
Vascular Endothelial Growth Factor A - genetics
Vehicles
X-rays
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Summary:Abstract Polyethylenimines (PEIs) are commonly used as a vehicle to deliver and protect siRNA, but the strong interaction still remains to be modulated for efficient siRNA release and silencing. Herein, a single-monomer derived linear-like PEI- co -PEG (LPEI- co -PEG, P2 ) was synthesized to substantially enhance the siRNA release, but not affect the efficiency of protection. The linear-like copolymer (P2 ) was only synthesized from a single-monomer by intensive synchrotron X-ray irradiation within 5 min, randomly producing both PEI and PEG segments. The counterpart vehicle, LPEI ( P1 ), was also synthesized for comparison. We found that the P1 and P2 were able to prevent siRNA against enzymatic degradation. Most importantly, efficient siRNA release (52%) was only observed in the siRNA/ P2 complexes and not in the siRNA/ P1 complexes (
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2011.01.059