Determination of organic acids in Vaccinium berry standard reference materials

• Published in: Analytical and bioanalytical chemistry 2010-09, Vol.398 (1), p.425-434
• Main Authors: Phillips, Melissa M, Case, Ryan J, Rimmer, Catherine A, Sander, Lane C, Sharpless, Katherine E, Wise, Stephen A, Yen, James H
• Format: Article
• Language: English
• Subjects: Acids - analysis; Acids - standards; Analytical Chemistry; Berry; Biochemistry; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Chromatographic methods and physical methods associated with chromatography; Chromatography, High Pressure Liquid; Chromatography, Reverse-Phase; Dietary supplements; Dietary Supplements - analysis; Dietary Supplements - standards; Electric properties; Electrochemical methods; Exact sciences and technology; Food Science; Fruit - chemistry; Gas chromatographic methods; gas chromatography; Gas Chromatography-Mass Spectrometry; ion exchange chromatography; isotope dilution technique; Isotope Labeling; Isotopes; Laboratory Medicine; Liquid chromatography; Mass Spectrometry; Monitoring/Environmental Analysis; Organic acids; organic acids and salts; Original Paper; Other chromatographic methods; Oxalates; Oxalic acid; Phosphates; Reference Standards; Solvent extraction; Spectrometric and optical methods; Standard reference materials; Sulfates; Ultraviolet; Vaccinium - chemistry
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-010-3916-0

Nine organic acids (citric acid, galacturonic acid, glycolic acid, isocitric acid, malic acid, oxalic acid, quinic acid, shikimic acid, and tartaric acid) and two anions (phosphate and sulfate) were determined in a suite of Vaccinium berry-containing dietary supplement standard reference materials (SRMs). Following solvent extraction, three independent methods were utilized in the quantification of these compounds. The first method involved reversed-phase liquid chromatography with ultraviolet absorbance detection at 210 nm and isotope dilution mass spectrometry. The second method utilized ion chromatography with conductivity detection. Finally, gas chromatography with isotope dilution mass spectrometry detection was used following derivatization with N-methyl-N-trifluoroacetamide (MSTFA). The combined data from these methods was used for the assignment of organic acid levels in the seven candidate SRMs.