Behavior of polysaccharide assemblies in field-flow fractionation and size-exclusion chromatography

Asymmetric flow field-flow fractionation (AsFlFFF) and high-performance size-exclusion chromatography (HPSEC) are techniques for separating and characterizing macromolecules; until now the latter is more utilized for analyzing polysaccharides. The demand for characterizing complex, high-molar-mass p...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2011-02, Vol.399 (4), p.1467-1472
Main Authors: Pitkänen, Leena, Tenkanen, Maija, Tuomainen, Päivi
Format: Article
Language:English
Subjects:
Aggregates
Analysis
Analytical Chemistry
Arabinoxylans
Asymmetric flow field-flow fractionation
Asymmetry
Biochemistry
Carbohydrates
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Chromatography
Chromatography, Gel
Comparative analysis
Distribution
Food Science
Fractionation
Fractionation, Field Flow - methods
Gel permeation chromatography
High performance liquid chromatography
Hydrodynamics
Hydrofoil boats
Laboratory Medicine
Mathematical analysis
Methods
Molecular weights
Monitoring/Environmental Analysis
Original Paper
Polysaccharides
Polysaccharides - analysis
Properties
Separation
Separation (Technology)
Separators (Machines)
Size-exclusion chromatography
Structure
Xylans
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Summary:Asymmetric flow field-flow fractionation (AsFlFFF) and high-performance size-exclusion chromatography (HPSEC) are techniques for separating and characterizing macromolecules; until now the latter is more utilized for analyzing polysaccharides. The demand for characterizing complex, high-molar-mass polysaccharides has raised interest in the use of AsFlFFF in analyzing polymeric carbohydrates in addition to HPSEC. In this paper, we compare the behavior of arabinoxylan aggregates present in aqueous solution in AsFlFFF and HPSEC and their effect on the obtained molecular characteristics (molar mass averages and size). Although the amount of aggregates in aqueous arabinoxylan solutions may be low, their role needs to be understood to avoid erroneous interpretations of AsFlFFF and HPSEC data. When these two separation systems were compared, AsFlFFF seemed to possess more separation power for the differentiation of aggregates from individual chains than HPSEC. To our knowledge, this is the first report on the characterization of xylans with AsFlFFF.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-010-4160-3