Identification of 14-3-3β in human gastric cancer cells and its potency as a diagnostic and prognostic biomarker

Gastric cancer is the second most common cause of cancer deaths worldwide and due to its poor prognosis, it is important that specific biomarkers are identified to enable its early detection. Through 2‐D gel electrophoresis and MALDI‐TOF‐TOF‐based proteomics approaches, we found that 14‐3‐3β, which...

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Published in:Proteomics (Weinheim) 2011-06, Vol.11 (12), p.2423-2439
Main Authors: Tseng, Chien-Wei, Yang, Jyh-Chin, Chen, Chiung-Nien, Huang, Hsuan-Cheng, Chuang, Kai-Neng, Lin, Chen-Ching, Lai, Hong-Shiee, Lee, Po-Huang, Chang, King-Jen, Juan, Hsueh-Fen
Format: Article
Language:English
Subjects:
14-3-3 Proteins - blood
14-3-3 Proteins - genetics
14-3-3β
Adult
Aged
Aged, 80 and over
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Biomarker
Biomarkers, Tumor - analysis
Biomarkers, Tumor - blood
Biomarkers, Tumor - genetics
Biomedicine
Cell Line, Tumor
Cell Movement - drug effects
Cell Proliferation - drug effects
Early Diagnosis
Electrophoresis, Gel, Two-Dimensional
Female
Fluorouracil - pharmacology
Fundamental and applied biological sciences. Psychology
Gastric cancer
Gastroenterology. Liver. Pancreas. Abdomen
Gene Expression Profiling
Humans
Lymphatic Metastasis - diagnosis
Lymphatic Metastasis - genetics
Lymphatic Metastasis - pathology
Male
Medical sciences
Middle Aged
Miscellaneous
Neoplasm Staging
Network analysis
Plasmids
Prognosis
Protein Interaction Mapping - methods
Proteins
Proteomics - methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Stomach - metabolism
Stomach - pathology
Stomach Neoplasms - blood
Stomach Neoplasms - diagnosis
Stomach Neoplasms - genetics
Stomach Neoplasms - mortality
Stomach Neoplasms - pathology
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Survival Analysis
Transfection
Tumors
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Summary:Gastric cancer is the second most common cause of cancer deaths worldwide and due to its poor prognosis, it is important that specific biomarkers are identified to enable its early detection. Through 2‐D gel electrophoresis and MALDI‐TOF‐TOF‐based proteomics approaches, we found that 14‐3‐3β, which was one of the proteins that were differentially expressed by 5‐fluorouracil‐treated gastric cancer SC‐M1 cells, was upregulated in gastric cancer cells. 14‐3‐3β levels in tissues and serum were further validated in gastric cancer patients and controls. The results showed that 14‐3‐3β levels were elevated in tumor tissues (n=40) in comparison to normal tissues (n=40; p
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.201000449