Structure elucidation of native N- and O-linked glycans by tandem mass spectrometry (tutorial)

Oligosaccharides play important roles in many biological processes. However, the structural elucidation of oligosaccharides remains a major challenge due to the complexities of their structures. Mass spectrometry provides a powerful method for determining oligosaccharide composition. Tandem mass spe...

Full description

Saved in:
Bibliographic Details
Published in:Mass spectrometry reviews 2011-07, Vol.30 (4), p.560-578
Main Authors: An, Hyun Joo, Lebrilla, Carlito B.
Format: Article
Language:English
Subjects:
Carbohydrate Conformation
Carbohydrate Sequence
CID
Glycopeptides - chemistry
Glycoproteins - chemistry
Infrared Rays
IRMPD
N-glycan
O-glycan
Oligosaccharides, Branched-Chain - chemistry
Spectroscopy, Fourier Transform Infrared
structure
tandem mass spectrometry
Tandem Mass Spectrometry - methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Oligosaccharides play important roles in many biological processes. However, the structural elucidation of oligosaccharides remains a major challenge due to the complexities of their structures. Mass spectrometry provides a powerful method for determining oligosaccharide composition. Tandem mass spectrometry (MS) provides structural information with high sensitivity. Oligosaccharide structures differ from other polymers such as peptides because of the large number of linkage combinations and branching. This complexity makes the analysis of oligosaccharide unique from that of peptides. This tutorial addresses the issue of spectral interpretation of tandem MS under conditions of collision‐induced dissociation (CID) and infrared multiphoton dissociation (IRMPD). The proper interpretation of tandem MS data can provide important structural information on different types of oligosaccharides including O‐ and N‐linked. © 2010 Wiley Periodicals, Inc., Mass Spec Rev 30:560–578, 2011
ISSN:0277-7037
1098-2787
DOI:10.1002/mas.20283