An in vitro screening method to evaluate chemicals as potential chemotherapeutants to control Aeromonas hydrophila infection in channel catfish

• Published in: Journal of applied microbiology 2011-07, Vol.111 (1), p.114-124
• Main Authors: Pridgeon, J.W., Klesius, P.H., Mu, X., Song, L.
• Format: Article
• Language: English
• Subjects: Aeromonas hydrophila; Animals; Bacteria - drug effects; Bacterial Infections - drug therapy; Bacterial Infections - microbiology; Bacterial Infections - veterinary; Biological and medical sciences; catfish; chemotherapeutant; Drug Evaluation, Preclinical - methods; Fish Diseases - drug therapy; Fish Diseases - microbiology; Fundamental and applied biological sciences. Psychology; gill cell; Gills - cytology; Gills - drug effects; hydrogen peroxide; Ictaluridae; in vitro screening; Microbiology
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/j.1365-2672.2011.05030.x

Aims:  To develop an in vitro screening method to be used for identifying potential effective chemotherapeutants to control Aeromonas hydrophila infections. Methods and Results:  Using catfish gill cells G1B and four chemicals (hydrogen peroxide, sodium chloride, potassium permanganate and d‐mannose), the feasibility of using an in vitro screening method to identify potential effective chemotherapeutants was evaluated in this study. In vitro screening results revealed that, at concentration of 100 mg l−1, H2O2 was the only chemical tested that was able to completely abolish the attachment and invasion of Aer. hydrophila to catfish gill cells. In vivo virulence studies using live channel catfish through bath immersion confirmed that H2O2 was the only chemical tested that was able to significantly (P