Evolutionary implications of substitution patterns in prolamin genes of Oryza glaberrima (African rice, Poaceae) and related species

Patterns of sequence variation of nuclear genes encoding 10-kDa and 16-kDa prolamin seed storage proteins were examined in Oryza glaberrima (African rice, Poaceae) and O. barthii and compared to available sequences for the genus to assess potential application of these gene families in evolutionary...

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Bibliographic Details
Published in:American journal of botany 2002-02, Vol.89 (2), p.211-219
Main Authors: Hilu, Khidir W., Sharova, Lioudmila V.
Format: Article
Language:English
Subjects:
Amino acids
Biological taxonomies
Botany
DNA
Evolution
Genes
Genetic mutation
Genetic variation
Genetics and Molecular Biology
molecular evolution
Oryza
Oryza barthii
Oryza glaberrima
Oryza longistaminata
Oryza rufipogon
Oryza sativa
Plants
Poaceae
Polymerase chain reaction
prolamin
prolamin genes
protein
Proteins
Pseudogenes
Rice
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Summary:Patterns of sequence variation of nuclear genes encoding 10-kDa and 16-kDa prolamin seed storage proteins were examined in Oryza glaberrima (African rice, Poaceae) and O. barthii and compared to available sequences for the genus to assess potential application of these gene families in evolutionary studies. Sequence variation among species in 10-kDa genes was very low. In contrast, the 16-kDa genes have undergone rapid evolution, displaying a larger number of length and point mutations that in some cases result in frame shift or produce truncated protein or pseudogenes. The proportion of nonsynonymous substitution is high in both genes. Although nonsynonymous mutations did not alter the overall profile of the protein, pronounced shifts in proportions of some amino acids were evident and could have systematic application. The data provide support for a proposed direct evolution of the Asian (O. sativa) and African rice from O. rufipogon and O. barthii, respectively. Patterns of amino acid frequencies of the 10-kDa genes show the distinctness of O. rufipogon and O. longistaminata from the other species. The study underscores the potential application of the prolamin genes as markers from the nuclear genome for evolutionary studies in grasses at different taxonomic levels.
ISSN:0002-9122
1537-2197
DOI:10.3732/ajb.89.2.211